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Chemistry Certification

Instructions:

Answer 50 questions in 15 minutes.
If you are not ready to take this test, you can study here.
Match each statement with the correct term.
Don't refresh. All questions and answers are randomly picked and ordered every time you load a test.

This is a study tool. The 3 wrong answers for each question are randomly chosen from answers to other questions. So, you might find at times the answers obvious, but you will see it re-enforces your understanding as you take the test each time.

1. The following data were obtained on amniotic fluid from a gestation of 36 weeks' duration by menstrual dates: L/S = 2 Phosphatidyl glycerol not present This can be concluded from these data?
L/S ratio > 3.5
oxalacetate to malate
the fetus is at risk for respiratory distress
SD over mean

2. A serum sample for acid phosphatase determination is best prepared for room temperature storage by...
standard
cysteine
adding an acid stabilizer
sodium

3. The enzyme that exists chiefly in skeletal muscle - heart - and brain; is grossly elevated in active muscular dystrophy; and rises early in myocardial infarction is...
creatine kinase
lead
fresh morning urine for delta-aminolevulinic acid
cyclic antidepressants

4. The measurement of light scattered by particles in the sample is the principle of...
creatinine clearance
nephelometry
TLC
CK

5. The most consistent analytical error involved in the routine determination of HDLcholesterol is caused by...
impaired response to ACTH stimulation
a small concentration of APO-B containing lipoproteins after precipitation
be able to detect 9/10 simple instrument malfunctions
peroxidase-like activity of hemoglobin

6. Before unconjugated bilirubin can react with Ehrlich's diazo reagent - this must be added
improving state of metabolic control (as indicated by fructosamine)
methyl alcohol
inclusion of smokers and nonsmokers in the study population
acute hepatitis

7. Amniotic fluid submitted for a lecithin/sphingomyelin (US) ratio has been left in a brown container since the night before. You would expect...
decreased lecithin
creatinine clearance
breast cancer
pH = pKa + log (salt/acid)

8. This is a glycolytic enzyme that catalyzes the cleavage of fructose-1 - 6-diphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate
100nmol
aldolase
porphobilinogen synthetase
5 tri 15 cholesterol 50 protein

9. Sixty to seventy-five percent of the plasma cholesterol is transported by...
B12 deficiency
quantitation of label
LDL
purulent

10. A 45-year-old woman complains of fatigue - heat intolerance - and hair loss. Serum thyroxine and 125I-T3 resin uptake are abnormally low. What test would confirm that this is a pituitary deficiency?
120 minutes
sample exposed to light
creatinine
TSH

11. The methodology based on the amount of energy absorbed by a subtance as a function of its concentration and using a specific source of the same material as the substance analyzed is...
parathyroid hormone
<45
serum cortisol > 15
atomic absorption

12. Stray light can be detected in a spectrophotometer by utilizing a...
Tangier
binding capacity of thyroxine-binding globulin
alpha 2 beta
sharp cutoff filter

13. A stool specimen that appears black and tarry should be tested for the presence of...
Hb S
thymolpthalein monophosphate
lithium
occult blood

14. Cocaine is metabolized to...
GGT
benzoylecogine
ratio of distance moved by solvent to distance moved by compound
respiratory acidosis

15. The nanometer is a measurement of...
9
accuracy
2Na + glucose over 20 + BUN over 3
wavelength

16. The urea nitrogen concentration of a serum sample was measured as 15 mg/dL (atomic weights: carbon = 12 - oxygen = 16 - nitrogen = 14 - hydrogen = 1). The urea concentration of the same sample - in mg/dL - is...
carbohydrate hyperalimentation
40-50
32
methadone

17. The formula for coefficient of variation?
acute hepatitis
SD over mean
parathyroid
didymium filter

18. The electrophoretic pattern of a plasma sample compared with a serum sample shows a...
accuracy
Mg
sharp fibrinogen peak
hepatobiliary (obstruction?)

19. Measurement of the serum acid phosphatase is used to detect neoplastic disease of the...
5mg higher
prostate
MI
ratio of distance moved by solvent to distance moved by compound

20. Detection of carriers of hereditary coproporphyria should include analysis of...
specificity
there is an absence of correlation
be able to detect 9/10 simple instrument malfunctions
fresh morning urine for delta-aminolevulinic acid

21. The osmolality of a urine or serum specimen is measured by a change in the...
GFR
Tangier
freezing point
glass

22. Certain enzymes are activated to metabolize drugs as a result of another drug action. This is referred to as...
chemical ionization matrix
is quantitated by the amount of light produced
measurement of absorbance at 450
enzyme induction

23. A serum potassium is 19.5 mg/100 mL. This value is equal to how many mEq/L (MW of K = 39)?
5
agarose
TSH
urinary free cortisol

24. Most automated blood gas analyzers directly measure...
pH pCO2 pO2
CK-MB
para-hydroxylphenyl...
ratio of distance moved by solvent to distance moved by compound

25. A thick white turbid specimen was received in the laboratory labeled as pericardial fluid. A microscopic examination was performed - and the differential included 90% PMNs. The fluid is...
specificity
Prolactin
parathyroid
purulent

26. A 45-year-old woman complains of fatigue - heat intolerance - and hair loss. Serum thyroxine and 125I-T3 resin uptake are abnormally low. What test would confirm that this is a pituitary deficiency?
changes in the fuel
TSH
thymolpthalein monophosphate
591

27. In the liver - bilirubin is converted to...
C3 complement
thyroxine
decreased lecithin
bilirubindiglucuronide

28. The anion gap is useful for quality control of laboratory results for...
sodium potassium chloride CO2
pO2 increased HCO3 decreased
bicarbonate carbonic acid
LDL

29. Currently the most common method for specific identification and quantitation of serum barbiturates is...
freezing point
endogenous triglycerides
GLC
SD over mean

30. Amino acid associated with a sulfhydryl group
L/S ratio > 3.5
oxalacetate to malate
placenta
cysteine

31. The buffering capacity of blood is maintained by a reversible exchange process between bicarbonate and...
5
chloride
atomic absorption
trig

32. Serum concentrations of vitamin B12 are elevated in...
CML
wavelength
sodium
mg/ml

33. Most chemical methods for determining total protein utilize this reaction?
beta
biuret
urobilin
iron deficiency anemia

34. Alkaline phosphatase Slight increase - Aminotransferase Marked increase - Aspartate aminotransferase Marked increase - Alanine gamma-glutamyl transferase Slight increase
lithium
LDH
acute hepatitis
endogenous triglycerides

35. Scurvy is associated with deficiency of...
dirty applicators
prevent phosphate interference
run a new set of controls
C

36. In using ion-exchange chromatographicbmethods - falsely increased levels of Hgb A1c will be demonstrated in presence of...
enzyme immunoassay
Hb S
amylase and lipase
be able to detect 9/10 simple instrument malfunctions

37. In automated methods utilizing a bichromatic analyzer - dual wavelengths are employed to...
carbohydrate hyperalimentation
4.5
minimize the effects of interference
specificity

38. The following laboratory results were obtained: Serum Calcium: Increased - Urine Calcium: Increased - Serum Phosphate: Decreased - Urine Phosphate: Increased Serum Alkaline Phosphatase: Normal or Increased These results are most compatible with...
bone
primary hyperparathyroidism
Temperature is a function of the number of particles in solution
faster than gamma

39. When separating serum proteins by cellulose acetate electrophoresis using veronal buffer at pH 8.6 - beta globulin migrates...
creatine kinase
faster than gamma
turn off
sample exposed to light

40. About 90% of phenytoin is excreted in the urine as...
para-hydroxylphenyl...
Hb S
valine leucine isoleucine
corticosterone

41. Upon completion of a run of cholesterol tests - the technician recognizes that the controls are not within the two standard deviations confidence range. What is the appropriate course of action
M subunit is inactivated
valine leucine isoleucine
run a new set of controls
200

42. An Rf value of 0.5 in thin-layer chromatography means...
show a decrease in glycosylated hgb
accuracy
5 tri 15 cholesterol 50 protein
Solute moves half the distance of the solvent front

43. Magnesium carbonate is added in an iron binding capacity determination in order to...
no decrease in serum growth hormone after 90 minutes
have a negative charge
bicarbonate carbonic acid
remove excess unbound iron

44. Following overnight fasting - hypoglycemia in adults is defined as a glucose of...
glass
oxalacetate to malate
thiamine
<45

45. An electrode has a silver/silver chloride anode and a platinum wire cathode. It is suspended in KCl solution and separated from the blood to be analyzed by a selectively permeable membrane. Such an electrode is used to measure...
tissue and serum levels need to reach equilibrium
turn off
diet
O2

46. Characteristic of the Bence Jones protein that is used to distinguish it from other urinary proteins is its solubility...
urinary free cortisol
@ 100
1 mol/kg
iron hemochromatosis

47. An increase in serum acetone is indicative of a defect in the metabolism of...
200
creatinine clearance
ionic charge
carbs

48. Pregnant women with symptoms of thirst - frequent urination - or unexplained weight loss should have this test performed?
Hb S
glycerol
cmpds based on molecular weight and size
glucose tolerance test

49. The reason carbon monoxide is so toxic is because it...
200x affinity for oxygen
run a new set of controls
elevated lactic acid - elevated pyruvate
Calcium - phosphorus

50. Which percentage of total serum calcium is nondiffusible protein bound?
acid phosphatase
effluent line is obstructed
40-50
volumetric flask