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Chemistry Certification
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Subject
:
certifications
Instructions:
Answer 50 questions in 15 minutes.
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Match each statement with the correct term.
Don't refresh. All questions and answers are randomly picked and ordered every time you load a test.
This is a study tool. The 3 wrong answers for each question are randomly chosen from answers to other questions. So, you might find at times the answers obvious, but you will see it re-enforces your understanding as you take the test each time.
1. Malic dehydrogenase is added to the aspartate aminotransaminase (AST) reaction to catalyze the conversion of...
inhibitor binds to enzyme at same site as substrate
Prolactin
oxalacetate to malate
quantitation of label
2. Patients with Cushing's syndrome exhibit...
C3 complement
serum cortisol > 15
have a negative charge
ionic charge
3. In electrophoresis of serum proteins - arifacts at the application point are most frequently caused by...
cancel test
dirty applicators
lactic acidosis
alkaline picrate
4. Upon completion of a run of cholesterol tests - the technician recognizes that the controls are not within the two standard deviations confidence range. What is the appropriate course of action
Mg
peptide bonds
serum cortisol > 15
run a new set of controls
5. In competitive inhibition of an enzyme reaction the...
oxalacetate to malate
inhibitor binds to enzyme at same site as substrate
accuracy
dirty electrodes
6. This enzyme substrates for prostatic acid phosphatase determination results in the highest specificity
increased vasoconstriction
TSH
thymolpthalein monophosphate
corticosterone
7. An HPLC operator notes that the column pressure is too high - is rising too rapidly - and the recorder output is not producing normal peaks. The most probable cause of the problem is...
200
improving state of metabolic control (as indicated by fructosamine)
Tangier
effluent line is obstructed
8. Which percentage of total serum calcium is nondiffusible protein bound?
M subunit is inactivated
properties of the membrane
40-50
aldolase
9. The most common type of congenital adrenal hyperplasia associated with increased plasma concentrations of 17 a-hydroxyprogesterone and increased urinary excretion of pregnanetriol is...
parathyroid
testosterone
21-hydroxylase deficiency
chylomicrons
10. Upon completion of a run of cholesterol tests - the technician recognizes that the controls are not within the two standard deviations confidence range. What is the appropriate course of action?
GLC
purulent
no decrease in serum growth hormone after 90 minutes
run a new set of controls
11. A condition in which erythrocyte protoporphyrin is increased is...
increased vasoconstriction
attaching them to protein molecules
TSH
iron deficiency anemia
12. A psychiatric patient was experiencing sever depression - but responded to treatment with amitriptyline with no apparent side effects. A blood sample was sent to the laboratory for therapeutic monitoring. Which drug level would be MOST reflective of
BUN increased
amytriptyline 104
oxalacetate to malate
alpha 2 beta
13. A patient with malabsorption receives 25 g of d-xylose orally; during the subsequent 5-hour period - the urine excretion of d-xylose is less than 3 g. This would indicate...
TSH
growth - third trimester of pregnancy
intestinal malabsorption
65
14. In familial hypercholesterolemia - the hallmark finding is an elevation of...
endogenous triglycerides
LDL
adding an acid stabilizer
primidone
15. A blood gas sample was sent to the lab on ice - and a bubble was present in the syringe. The blood had been exposed to room air for at least 30 minutes. The following change in blood gases occurred...
the fetus is at risk for respiratory distress
remove excess unbound iron
increased O2 decreased HCO3
metabolic acidosis
16. A spectrophotometer is being considered for purchase by a small laboratory. This specification reflects the spectral purity of the instrument
turn off
transferrin
diet
band width
17. Spectrophotometers isolate a narrow band pass by means of...
lead
creatine kinase
ALP with fresh draw
prisms and grating
18. Bioavailability of a drug refers to the...
increased O2 decreased HCO3
bone
fraction absorbed into systemic circulation
270
19. A serum sample for acid phosphatase determination is best prepared for room temperature storage by...
cyclic antidepressants
impaired response to ACTH stimulation
adding an acid stabilizer
SD over mean
20. The following results were obtained in a creatinine clearance evaluation: Urine concentration 84 mg/dL Urine volume 1440 mL/24 hr Serum concentration 1.4 mg/dL Body surface area 1.60 m2 (average = 1.73 m2) The creatinine cle~trance in mL/min is...
128
thyroxine binding globulin
65
hyperthyroidism
21. A patient with type I insulin-dependent diabetes mellitus has the following results: Fasting blood glucose: 150 mg/dL (70-110 rng/dL) Hemoglobin A1c: 25% (6.0%-19.5%) Fructosamine: 3.0 mmol/L ( 2.0-5.3 mmol/L)
respiratory ACIDOSIS
nephelometry
improving state of metabolic control (as indicated by fructosamine)
HDL
22. Gel filtration chromatography is used to separate...
freezing point
TSH
BUN 80
cmpds based on molecular weight and size
23. A breakdown product of hemoglobin is...
exposure to CO
bilirubin
sample exposed to light
accuracy
24. This wavelengths is within the ultraviolet rang.
BUN 80
low pH
porphobilinogen synthetase
340
25. The osmolal gap is defined as measured Osm/kg minus the calculated Osm/kg. The average osmolal gap is near...
fetal and placenta function
MI
0
5 tri 15 cholesterol 50 protein
26. Lasting serum phosphate concentration is controlled primarily by the...
parathyroid
be able to detect 9/10 simple instrument malfunctions
growth - third trimester of pregnancy
nephelometry
27. A 2-year-old child with a decreased serum T4 is described as being somewhat dwarfed - stocky - overweight - and having coarse features. Of the following - the most informative additional laboratory test would be the serum...
correct for variations in light intensity
Solute moves half the distance of the solvent front
serum aliquots - one heated
TSH
28. The direction in which albumin migrates (ie - toward anode or cathode) during electrophoretic separation of serum proteins - at pH 8.6 - is determined by...
plasma bicarbonate
testing heparinized plasma
iron deficiency anemia
ionization of carboxyl groups (yielding a net negative charge)
29. Total iron-binding capacity measures the serum iron transporting capacity of...
amylase
100nmol
nondiagnostic for MS
transferrin
30. In the immunoinhibition phase of the CK-MB procedure...
binding capacity of thyroxine-binding globulin
ratio of distance moved by solvent to distance moved by compound
iron deficiency anemia
M subunit is inactivated
31. Most chemical methods for determining total protein utilize this reaction?
LDL
cancel test
biuret
C3 complement
32. The formula for calculating absorbance given the percent transmittance (%T) of a solution
plasma bicarbonate
2-log(T)
cmpds based on molecular weight and size
creatinine
33. Given the following values:100;120;150;140;130;What is the mean?
hepatobiliary (obstruction?)
attaching them to protein molecules
128
improving state of metabolic control (as indicated by fructosamine)
34. The glucose level in normal peritoneal fluid should be...
the same as plasma glucose
volumetric flask
creatinine clearance
40-50
35. Isoenzyme assays are preformed to improve...
exposure to CO
acute intermittent porphyria
porphobilinigen SCREEN
specificity
36. The most widely employed screening technique for drug abuse is...
TLC
chemical ionization matrix
ratio of distance moved by solvent to distance moved by compound
purulent
37. A cardiac glycoside that is used in the treatment of congenital heart failure and arrhythmias by increasing the force and velocity of myocardial contraction is...
1 mol/kg
digoxin
fresh morning urine for delta-aminolevulinic acid
peroxidase-like activity of hemoglobin
38. The anticonvulsant used to control tonic-clonic (grand mal) seizures is...
ionization of carboxyl groups (yielding a net negative charge)
hemolysis
creatine kinase
phenytoin
39. An Rf value of 0.5 in thin-layer chromatography means...
high carbohydrate diet for 3 days
specificity
Solute moves half the distance of the solvent front
lead
40. This elevates carboxyhemoglobin?
exposure to CO
NADH
carbohydrate hyperalimentation
properties of the membrane
41. The formula for calculating serum osmolality that incorporates a correction for the water content of plasma is...
lipase
2Na + glucose over 20 + BUN over 3
200x affinity for oxygen
biuret
42. In respiratory acidosis - a compensatory mechanism is the increase in...
viral hepatitis
dirty applicators
run a new set of controls
plasma bicarbonate
43. Amniotic fluid submitted for a lecithin/sphingomyelin (US) ratio has been left in a brown container since the night before. You would expect...
1.1
decreased lecithin
bicarbonate buffer
increased O2 decreased HCO3
44. The most heat labile fraction of alkaline phosphatase is obtained from...
prisms and grating
bone
Wilson's disease
parathyroid
45. A patient in her 33rd week of pregnancy is hospitalized with toxemia. Her doctor would like to deliver the baby early because the toxemia is becoming more severe. The baby's best chance of delivery without respiratory distress due to lung immaturity
hyperthyroidism
L/S ratio > 3.5
normal - liver breakdown
nondiagnostic for MS
46. A new method is being evaluated. A recovery experiment is performed with the following results: 0.9 mL serum sample + 0.1 mL H20 = 89 mEq/L 0.9 mL serum sample + 0.1 mL analyte std. @ 800 mEq/L = 161 mEq/L The percent recovery of the added analyt
90%
ionization of carboxyl groups (yielding a net negative charge)
A1c
cysteine
47. Gas chromatography with the nitrogen/phosphorus detector is the most commonly used technique for the analysis of...
cyclic antidepressants
120 minutes
measures one ion more than others
lead
48. The chemical composition of HDL- cholesterol corresponds to...
triglycerides
5 tri 15 cholesterol 50 protein
Solute moves half the distance of the solvent front
one micromole/minute
49. The following five sodium control values (mEq/L) were obtained:140 - 135 - 138 - 140 - 142Calculate the coefficient of variation.
acid phosphatase
1.9
40-50
one micromole/minute
50. Following overnight fasting - hypoglycemia in adults is defined as a glucose of...
<45
viral hepatitis
2Na + glucose over 20 + BUN over 3
21-hydroxylase deficiency
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