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Chemistry Certification

Instructions:

Answer 50 questions in 15 minutes.
If you are not ready to take this test, you can study here.
Match each statement with the correct term.
Don't refresh. All questions and answers are randomly picked and ordered every time you load a test.

This is a study tool. The 3 wrong answers for each question are randomly chosen from answers to other questions. So, you might find at times the answers obvious, but you will see it re-enforces your understanding as you take the test each time.

1. In performing a spinal fluid protein determination - the specimen is diluted 1 part spinal fluid to 3 parts saline to obtain a result low enough to measure. To calculate the protein concentration the result must be...
multiplied by 4
immunofixation
spun separated iced and tested immediately
apoenzyme

2. The unit of measure for a standard solution is...
a small concentration of APO-B containing lipoproteins after precipitation
5mg higher
mg/ml
parathyroid hormone

3. The definitive diagnosis of primary adrenal insufficiency requires demonstration of...
agarose
impaired response to ACTH stimulation
correct for variations in light intensity
4.5

4. As part of a hyperlipidemia screening program - the following results were obtained on a 25 year old woman 6 hours after eating:Triglycerides = 260 mg/dLCholesterol = 120 mg/dL - This is the BEST interpretation of these results?
cholesterol normal triglycerides elevated
be able to detect 9/10 simple instrument malfunctions
lead
BUN 80

5. The direction in which albumin migrates (ie - toward anode or cathode) during electrophoretic separation of serum proteins - at pH 8.6 - is determined by...
remove excess unbound iron
specimen exposure to light
Wilson's disease
ionization of carboxyl groups (yielding a net negative charge)

6. The reason carbon monoxide is so toxic is because it...
200x affinity for oxygen
viral hepatitis
bone
carbohydrate hyperalimentation

7. A new method is being evaluated. A recovery experiment is performed with the following results: 0.9 mL serum sample + 0.1 mL H20 = 89 mEq/L 0.9 mL serum sample + 0.1 mL analyte std. @ 800 mEq/L = 161 mEq/L The percent recovery of the added analyt
90%
200x affinity for oxygen
amylase
didymium filter

8. Lasting serum phosphate concentration is controlled primarily by the...
M subunit is inactivated
pituitary
parathyroid
128

9. At a pH of 8.6 the gamma globulins move toward the cathode - despite the fact that they are negatively charged. What is this phenomenon called?
endosmosis
iron deficiency anemia
specificity
spun separated iced and tested immediately

10. This serum protein migrates with the beta-globulins on cellulose acetate at pH 8.6...
C3 complement
benzoylecogine
patient noncompliance
A1

11. Acidosis and alkalosis are best defined as fluctuations in blood pH and CO2 content due to changes in...
increased vasoconstriction
bicarbonate buffer
Hb S
wavelength

12. The hemoglobin that is resistant to alkali (KOH) denaturation is...
coulometry
F
biuret
LDL

13. Following overnight fasting - hypoglycemia in adults is defined as a glucose of...
<45
biuret
benzoylecogine
MI

14. A stool specimen that appears black and tarry should be tested for the presence of...
bicarbonate buffer
correct for variations in light intensity
freezing point
occult blood

15. About 90% of phenytoin is excreted in the urine as...
TSH
para-hydroxylphenyl...
2-log(T)
brain

16. The most important buffer pair in plasma is the...
bicarbonate carbonic acid
glucose
1:5
absorptivity x light path x concentration

17. This chemical determination may be of help in establishing the presence of seminal fluid
9
immunofixation
pituitary
acid phosphatase

18. A sweat chloride result of 55 mEq/L and a sweat sodium of 52 mEq/L were obtained ona patient who has a history of respiratory problems. The bestc interpretation of these results is...
intestinal malabsorption
sharp cutoff filter
boderline results test should be repeated
increased O2 decreased HCO3

19. A one MOLAL solution is equivalent to...
didymium filter
incorrect aqueous to nonaqueous mixture
1 mol/kg
HCT

20. An HPLC operator notes that the column pressure is too high - is rising too rapidly - and the recorder output is not producing normal peaks. The most probable cause of the problem is...
occult blood
effluent line is obstructed
para-hydroxylphenyl...
catalyze redox reactions

21. In the assay of lactate dehydrogenase - this product is actually measured
serum cortisol > 15
maintain colloidal osmotic pressure
NADH
5 tri 15 cholesterol 50 protein

22. Serum from a patient with metastatic carcinoma of the prostate was separated from the clot and stored at room temperature. The following results were obtained: Patient Reference Range Calcium: 10.8 mg/dL 8.8-10.3 mg/dL LD:420 U/L 50-150 U/L Acid phos
benzoylecogine
21-hydroxylase deficiency
monitoring the course of a known cancer
ALP with fresh draw

23. A cardiac glycoside that is used in the treatment of congenital heart failure and arrhythmias by increasing the force and velocity of myocardial contraction is...
digoxin
TSH
alpha 2 beta
1.1

24. Creatinine clearance is used to estimate the...
low pH
GFR
enzyme immunoassay
turn off

25. Calcium concentration in the serum is regulated by...
volumetric flask
tissue and serum levels need to reach equilibrium
parathyroid hormone
minimize the effects of interference

26. Bile acid concentrations are useful to assess...
hepatobiliary (obstruction?)
glass
changes in the fuel
can be used for absorbtion - partition - ion exchange - and gel permeation chromatography

27. Diagnostic specificity is defined as the percentage of individuals...
porphobilinigen SCREEN
normal - liver breakdown
WITHOUT a disease with a negative test
cyclic antidepressants

28. Currently the most common method for specific identification and quantitation of serum barbiturates is...
ionization of carboxyl groups (yielding a net negative charge)
spun separated iced and tested immediately
transferrin
GLC

29. A blood gas sample was sent to the lab on ice - and a bubble was present in the syringe. The blood had been exposed to room air for at least 30 minutes. The following change in blood gases occurred...
Tangier
lactic acidosis
pO2 increased HCO3 decreased
5

30. The most widely used support medium for electrophoretic separation of lipoproteins is...
agarose
<1%
testing heparinized plasma
128

31. The T3 resin uptake test is a measure of...
binding capacity of thyroxine-binding globulin
plasma bicarbonate
5
g/MW

32. A serum sample for acid phosphatase determination is best prepared for room temperature storage by...
lead
tissue and serum levels need to reach equilibrium
analyzed immediately
adding an acid stabilizer

33. The serum glucose concentration of a diabetic patient undergoing a 2-hour glucose tolerance test should return to the baseline (fasting level) after a minimum of...
GFR
5
120 minutes
cancel test

34. Malic dehydrogenase is added to the aspartate aminotransaminase (AST) reaction to catalyze the conversion of...
oxalacetate to malate
decreased lecithin
5mg higher
1.1

35. Hydrogen ion concentration (pH) in blood is usually determined by means of these electrodes?
thiamine
glass
bilirubindiglucuronide
measure the total volume

36. Specimens for blood gas determination should be drawn into a syringe containing...
A1
thymolpthalein monophosphate
normal - liver breakdown
heparin

37. This wavelengths is within the ultraviolet rang.
changes in the fuel
340
sample exposed to light
1 mol/kg

38. Regan isoenzyme has the same properties as alkaline phosphatase that originates in the...
tissue and serum levels need to reach equilibrium
binding capacity of thyroxine-binding globulin
TSH
placenta

39. Sixty to seventy-five percent of the plasma cholesterol is transported by...
act as carriers for ions
LDL
HDL
correct for variations in flame and atomizer

40. In a normal individual - injection of thyrotropin-releasing hormone (TRH) causes an increase in blood concentrations of this hormone
Prolactin
boderline results test should be repeated
diet
porphobilinogen screen

41. Aminotransferase enzymes catalyze the...
M subunit is inactivated
100nmol
T4 by RIA
exchange of amino and keto groups

42. Four children are admitted with malaise - anorexia - and abdominal pain. Further evaluations reveal mild anemia - erythrocyte basophilic stippling - and profound pica habits. Poisoning by which heavy metal is most likely responsible?
cysteine
0
lead
high co2 and hco3

43. What substance gives feces its normal color?
exchange of amino and keto groups
pH = pKa + log (salt/acid)
measure the total volume
urobilin

44. Zinc protoporphyrin or free erythrocyte protoporphyrin measurements are useful to assess blood concentrations of...
128
lead
MI
the fetus is at risk for respiratory distress

45. The principle of the occult blood test depends on the...
binding capacity of thyroxine-binding globulin
biuret
peroxidase-like activity of hemoglobin
heparin

46. Quantitation of NA+ and K+ by ion-selective electrode is more rapid than flame photometry because...
sample exposed to light
whole blood can be used
growth - third trimester of pregnancy
multiplied by 4

47. A patient is admitted to the emergency room in a state of metabolic alkalosis.This would be consistent with this diagnosis?
pH pCO2 pO2
high co2 and hco3
check serum for hemolysis
TSH

48. If the correlation coefficient (r) of two variables is zero...
(testing of urine for) delta-am.. acid
there is an absence of correlation
LDL
serum aliquots - one heated

49. The following data were obtained on amniotic fluid from a gestation of 36 weeks' duration by menstrual dates: L/S = 2 Phosphatidyl glycerol not present This can be concluded from these data?
prisms and grating
pH pCO2 pO2
the fetus is at risk for respiratory distress
enzyme immunoassay

50. The identification of Bence Jones protein is best accomplished by...
no decrease in serum growth hormone after 90 minutes
hyperventilation
immunofixation
glycerol