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Genetic Engineering Biotechnology

Subject : engineering
Instructions:
  • Answer 50 questions in 15 minutes.
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  • Match each statement with the correct term.
  • Don't refresh. All questions and answers are randomly picked and ordered every time you load a test.

This is a study tool. The 3 wrong answers for each question are randomly chosen from answers to other questions. So, you might find at times the answers obvious, but you will see it re-enforces your understanding as you take the test each time.
1. Gene therapy involves insertion of particular recombinant DNA sequences that encode for ___________; used increasingly to replace missing or mutated genes to correct inherited defects






2. Many individuals recieving treatment for their Hemophilia A through blood transfusions contracted the HIV virus - through the donated plasma






3. A lack of an essential hormone causes the disease and treatment involves replacing the missing hormone






4. Ethyl alcohol






5. Are observable variations in DNA which help establish a profile






6. Pieces of DNA produced by endonucleases - when in the laboratory - endonucleases are used to cleave DNA at desired sites - allowing them to be seperated by Gel Electrophoresis by size - then allowing the nucleotide sequences to be isolated and ident






7. A good recombinant vector must be capable of carrying a significant fragment of DNA and readily accept it






8. Is a lab technique used to separate various sized restriction fragments






9. Involves manipulation of an organism's genome - Used in conjunction with biotechnology - which involves practical use of an organism's biochemical and metabolic pathways for the production of food products - chemicals - drugs andother products






10. Was a 4 yr old girl - in 1990 suffering from severe immunodeficiency disease caused by lack of enzyme adenosine deaminase (ADA). Researchers at the National Institutes of Health - transfused her own blood cells that had been engineered to contain the






11. After the actual size of individual fragments are determined - the nucleotide sequence which has been isolated and identified - can be inserted into an acceptable cloning host through the process of transfection






12. To deliberately remove genetic material from one organism - and combine it with the genetic material of another






13. Inherent differences in ________ of DNA are used to identify humans - animals - and microbes;DNA analysis has revolutionized fields such as epidemiology and criminalistics; DNA technology is now common - place in forensic investigations






14. Identification of the gene responsible for the defective or absent protein - identification - isolation - cloning of the normal gene and transfection into the cells of the individual with the disease.






15. Used in industry - agriculture - food sciences and environmental protection. Some utilize genetic engineering techniques to achieve a desired outcome or rely on natu4ral biochemical and metabolic activities of organisms






16. yeasts ferment carbs in fruits & grains anaerobically resulting in producing ethyl alcohol






17. Fast growth rate - ability to be grown in large quantities using ordinary culture methods - Nonpathogenic -Genome that is well- delineated (mapped) - capable of accepting plasmids or bacteriophage vectors - maintains foreign genes through multiple ge






18. Gel electrophoresis is a process based on migration of negatively charged DNA fragments through a gel - toward a positive electrode






19. Recombinant DNA may enable __________ to determine the likelihood of a praticular genetic disease long before the appearance of symptoms associated with the disease;early detection may allow intervention before the occurrence of irreversible damage.






20. Use of an organism's biochemical processes to produce a needed product - These processes have been greatly enhanced by use of recombinant DNA which allows organisms to be genetically modified to accomplish tasks that were previously not possible






21. Plasmids and bacteriophages






22. Are recombinant organisms produced by insertion of recombinant DNA fragments






23. Profile established by analysis of multiple markers provides unique characteristic of individual whose DNA is presented;






24. Gene therapy






25. Are bacterial enzymes that recognize foreign DNA and are capable of breaking the bonds between adjacent nucleotides; each has a specific nucleotide sequence as its target






26. Once the specific functional protein will be synthesized - the availbility of the protein will eliminate the disease condition






27. Derived from potatos is the most widely marketed and consumed alcoholic beverage






28. DNA fingerprinting of specimens that contain only minute amounts of DNA or even old specimens that may not be suitable for other test methods






29. Incorportates air into the dough






30. Forensic medicin; examples : fingerprints - hair - skin - fingernails - semen - blood or saliva






31. Enzyme required for conversion of inactive protein plasminogen to its active form plasmin; is used to dissolve clots associated with myocardial and cerebral infarctions - availablity of tPA from human origin is limitied and not sufficient to meet the






32. Incompatible DNA of bacteriophages and plasmids






33. Recombinant DNA may be inserted into ________ to produce organisms that are better adapted to their environments - may not be disease or drought tolerant - may provide more nutritional properties - may be easier to grow - or may even convey immunity






34. Saccharomyctes cervisiae

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35. Caused by a lack of adequate Human Growth Hormone (HGH); once treated by harvesting HGH from the pituitary glands of human cadavers






36. Degree of uniqueness is directly proportional to the prevalence of particular markers in comparison population; use of multiple markers allow significant increase in actual uniqueness of profile established






37. Can revert to anaerobic fermentation in the event they have exhausted their oxygen supply - creating lactic acid






38. The actual sequence of nucleotides that make up the DNA molecule; we have due to lab techniques in genetic engineering - the ability to creat a DNA fingerprint ( DNA type or DNA profile) that is unique identifying characteristic of an individual






39. A faulty gene in humans suffering from a disease which is due to the lack of a protein or the presence of a defective protein is corrected or reparied; inherent benefit is to permanently cure physiological dysfunction by repairing a genetic defect






40. Enabling the large- scale production of life- saving or life- altering hormones and enzymes of human origin






41. Caused by a lack of adequate clotting factor VIII;






42. Anaerobic fermentation in skeletal muscles produces






43. Corn mash whiskey - is clear after distillation - the aging process while in an oak barrel for years - is responsible for the golden color and distinct taste of bourbon (or Jack Daniels Tennessee whiskey)






44. Detecting variations in DNA sequence at same location in genome of different individuals;






45. Although the availability of HGH was limited at best - demand exceeding the opportunity of supply - the potential transmission of infectious agents such as the prion associated with Creutzfedt- Jacob disease was life threatening to the individual rec






46. Final product of recombinant DNA may be production of a particular protein such as _______ - human growth factor and certain clotting factors; recombinant DNA is transferred into bacteria - which can utilize routine protein synthesis mechanisms to pr






47. Can be inserted into plants or animals to produce organisms that are better adapted to their environments - has been shown to cause improvements in crops by improving things such as drought resistance and has conveyed immunity to those consuming them






48. Actions of microbial enzymes that break down flour proteins ( gluten) and give dough its elasticity






49. The process of introducing foreign genes into a cloning host organism






50. To detect presence of genes that increase risk of hereditary diseases and establish patterns of inheritance of the diseases; it can be used to establish paternity and maternity - genetic ancestry of domestic animals an genetic diversity of animals br