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Genetic Engineering Biotechnology

Subject : engineering
Instructions:
  • Answer 50 questions in 15 minutes.
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  • Match each statement with the correct term.
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This is a study tool. The 3 wrong answers for each question are randomly chosen from answers to other questions. So, you might find at times the answers obvious, but you will see it re-enforces your understanding as you take the test each time.
1. yeasts ferment carbs in fruits & grains anaerobically resulting in producing ethyl alcohol






2. Migration is due to attraction of opposite charges - smaller fragments move faster than larger ones - which allows the restriction fragments to be not just seperated but isolated and identified






3. Gel electrophoresis is a process based on migration of negatively charged DNA fragments through a gel - toward a positive electrode






4. Although the availability of HGH was limited at best - demand exceeding the opportunity of supply - the potential transmission of infectious agents such as the prion associated with Creutzfedt- Jacob disease was life threatening to the individual rec






5. Plasmids and bacteriophages






6. Ethyl alcohol






7. Involves manipulation of an organism's genome - Used in conjunction with biotechnology - which involves practical use of an organism's biochemical and metabolic pathways for the production of food products - chemicals - drugs andother products






8. Was a 4 yr old girl - in 1990 suffering from severe immunodeficiency disease caused by lack of enzyme adenosine deaminase (ADA). Researchers at the National Institutes of Health - transfused her own blood cells that had been engineered to contain the






9. Once the specific functional protein will be synthesized - the availbility of the protein will eliminate the disease condition






10. Genetic Engineering is based on the ability to manipulate DNA by using a variety of ___________;allow DNA to cut precisely determined points along each strand - to be separated according to size of fragments and to be sequenced to determine exact com






11. Identification of the gene responsible for the defective or absent protein - identification - isolation - cloning of the normal gene and transfection into the cells of the individual with the disease.






12. The actual sequence of nucleotides that make up the DNA molecule; we have due to lab techniques in genetic engineering - the ability to creat a DNA fingerprint ( DNA type or DNA profile) that is unique identifying characteristic of an individual






13. Incompatible DNA of bacteriophages and plasmids






14. A good recombinant vector must be capable of carrying a significant fragment of DNA and readily accept it






15. Limited availablity of the essential - missing or deficient hormone - which can be aided by pharmaceutical companies use of recombinant DNA technology.






16. Caused by a lack of adequate Human Growth Hormone (HGH); once treated by harvesting HGH from the pituitary glands of human cadavers






17. Derived from potatos is the most widely marketed and consumed alcoholic beverage






18. A faulty gene in humans suffering from a disease which is due to the lack of a protein or the presence of a defective protein is corrected or reparied; inherent benefit is to permanently cure physiological dysfunction by repairing a genetic defect






19. Anaerobic fermentation in skeletal muscles produces






20. Recombinant DNA may be inserted into ________ to produce organisms that are better adapted to their environments - may not be disease or drought tolerant - may provide more nutritional properties - may be easier to grow - or may even convey immunity






21. Enabling the large- scale production of life- saving or life- altering hormones and enzymes of human origin






22. Insertion of particular recombinant DNA sequences that encode missing or dysfunctional proteins






23. Final product of recombinant DNA may be production of a particular protein such as _______ - human growth factor and certain clotting factors; recombinant DNA is transferred into bacteria - which can utilize routine protein synthesis mechanisms to pr






24. Incorportates air into the dough






25. Pieces of DNA produced by endonucleases - when in the laboratory - endonucleases are used to cleave DNA at desired sites - allowing them to be seperated by Gel Electrophoresis by size - then allowing the nucleotide sequences to be isolated and ident






26. Derived from sugar cane juice - is a primary alcoholic product of the caribbean islands - can be purchased in several different concentrations






27. Can revert to anaerobic fermentation in the event they have exhausted their oxygen supply - creating lactic acid






28. Saccharomyctes cervisiae

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29. A lack of an essential hormone causes the disease and treatment involves replacing the missing hormone






30. Use of an organism's biochemical processes to produce a needed product - These processes have been greatly enhanced by use of recombinant DNA which allows organisms to be genetically modified to accomplish tasks that were previously not possible






31. Profile established by analysis of multiple markers provides unique characteristic of individual whose DNA is presented;






32. Actions of microbial enzymes that break down flour proteins ( gluten) and give dough its elasticity






33. Corn mash whiskey - is clear after distillation - the aging process while in an oak barrel for years - is responsible for the golden color and distinct taste of bourbon (or Jack Daniels Tennessee whiskey)






34. Gene therapy involves insertion of particular recombinant DNA sequences that encode for ___________; used increasingly to replace missing or mutated genes to correct inherited defects






35. Recombinant DNA may enable __________ to determine the likelihood of a praticular genetic disease long before the appearance of symptoms associated with the disease;early detection may allow intervention before the occurrence of irreversible damage.






36. Forensic medicin; examples : fingerprints - hair - skin - fingernails - semen - blood or saliva






37. Use recombinant DNA technology to synthesize products that cannot be manufactured by other methods - or cannot be manufactured as efficiently or effectively by other methods






38. Which encodes for a particular protein product into the genome of appropriate host organism transforms the host organism into a production plant for the protein that is the genetic expression of a particular sequence - then - by means of routine bioc






39. Is essential in the process of clot dissolution associated with myocardial and cerebral infarctions






40. Are observable variations in DNA which help establish a profile






41. The process of introducing foreign genes into a cloning host organism






42. Used in industry - agriculture - food sciences and environmental protection. Some utilize genetic engineering techniques to achieve a desired outcome or rely on natu4ral biochemical and metabolic activities of organisms






43. Lactobacillus Sanfrancisco






44. Involves removal of a selected gene from on organism (genetic donor) followed by its propagation in a different organism (cloning host)






45. Gene therapy






46. Is a lab technique used to separate various sized restriction fragments






47. Detecting variations in DNA sequence at same location in genome of different individuals;






48. Caused by a lack of adequate clotting factor VIII;






49. Inherent differences in ________ of DNA are used to identify humans - animals - and microbes;DNA analysis has revolutionized fields such as epidemiology and criminalistics; DNA technology is now common - place in forensic investigations






50. Enzyme required for conversion of inactive protein plasminogen to its active form plasmin; is used to dissolve clots associated with myocardial and cerebral infarctions - availablity of tPA from human origin is limitied and not sufficient to meet the







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