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Genetic Engineering Biotechnology

Subject : engineering
Instructions:
  • Answer 50 questions in 15 minutes.
  • If you are not ready to take this test, you can study here.
  • Match each statement with the correct term.
  • Don't refresh. All questions and answers are randomly picked and ordered every time you load a test.

This is a study tool. The 3 wrong answers for each question are randomly chosen from answers to other questions. So, you might find at times the answers obvious, but you will see it re-enforces your understanding as you take the test each time.
1. Fast growth rate - ability to be grown in large quantities using ordinary culture methods - Nonpathogenic -Genome that is well- delineated (mapped) - capable of accepting plasmids or bacteriophage vectors - maintains foreign genes through multiple ge






2. Identification of the gene responsible for the defective or absent protein - identification - isolation - cloning of the normal gene and transfection into the cells of the individual with the disease.






3. Can revert to anaerobic fermentation in the event they have exhausted their oxygen supply - creating lactic acid






4. To detect presence of genes that increase risk of hereditary diseases and establish patterns of inheritance of the diseases; it can be used to establish paternity and maternity - genetic ancestry of domestic animals an genetic diversity of animals br






5. The process of introducing foreign genes into a cloning host organism






6. Are recombinant organisms produced by insertion of recombinant DNA fragments






7. Use of an organism's biochemical processes to produce a needed product - These processes have been greatly enhanced by use of recombinant DNA which allows organisms to be genetically modified to accomplish tasks that were previously not possible






8. Limited availablity of the essential - missing or deficient hormone - which can be aided by pharmaceutical companies use of recombinant DNA technology.






9. Are observable variations in DNA which help establish a profile






10. Profile established by analysis of multiple markers provides unique characteristic of individual whose DNA is presented;






11. DNA fingerprinting of specimens that contain only minute amounts of DNA or even old specimens that may not be suitable for other test methods






12. yeasts ferment carbs in fruits & grains anaerobically resulting in producing ethyl alcohol






13. Involves manipulation of an organism's genome - Used in conjunction with biotechnology - which involves practical use of an organism's biochemical and metabolic pathways for the production of food products - chemicals - drugs andother products






14. Incompatible DNA of bacteriophages and plasmids






15. Recombinant DNA may be inserted into ________ to produce organisms that are better adapted to their environments - may not be disease or drought tolerant - may provide more nutritional properties - may be easier to grow - or may even convey immunity






16. Insertion of particular recombinant DNA sequences that encode missing or dysfunctional proteins






17. Which encodes for a particular protein product into the genome of appropriate host organism transforms the host organism into a production plant for the protein that is the genetic expression of a particular sequence - then - by means of routine bioc






18. Caused by a lack of adequate Human Growth Hormone (HGH); once treated by harvesting HGH from the pituitary glands of human cadavers






19. A faulty gene in humans suffering from a disease which is due to the lack of a protein or the presence of a defective protein is corrected or reparied; inherent benefit is to permanently cure physiological dysfunction by repairing a genetic defect






20. Used in industry - agriculture - food sciences and environmental protection. Some utilize genetic engineering techniques to achieve a desired outcome or rely on natu4ral biochemical and metabolic activities of organisms






21. Degree of uniqueness is directly proportional to the prevalence of particular markers in comparison population; use of multiple markers allow significant increase in actual uniqueness of profile established






22. Caused by a lack of adequate insulin production - once treated using insulin derived from animals






23. Nucleotide sequences of DNA






24. Gel electrophoresis is a process based on migration of negatively charged DNA fragments through a gel - toward a positive electrode






25. Although the availability of HGH was limited at best - demand exceeding the opportunity of supply - the potential transmission of infectious agents such as the prion associated with Creutzfedt- Jacob disease was life threatening to the individual rec






26. Use recombinant DNA technology to synthesize products that cannot be manufactured by other methods - or cannot be manufactured as efficiently or effectively by other methods






27. Plasmids and bacteriophages






28. Migration is due to attraction of opposite charges - smaller fragments move faster than larger ones - which allows the restriction fragments to be not just seperated but isolated and identified






29. Enzyme required for conversion of inactive protein plasminogen to its active form plasmin; is used to dissolve clots associated with myocardial and cerebral infarctions - availablity of tPA from human origin is limitied and not sufficient to meet the






30. Once the specific functional protein will be synthesized - the availbility of the protein will eliminate the disease condition






31. Many individuals recieving treatment for their Hemophilia A through blood transfusions contracted the HIV virus - through the donated plasma






32. Forensic medicin; examples : fingerprints - hair - skin - fingernails - semen - blood or saliva






33. Recombinant DNA may enable __________ to determine the likelihood of a praticular genetic disease long before the appearance of symptoms associated with the disease;early detection may allow intervention before the occurrence of irreversible damage.






34. A lack of an essential hormone causes the disease and treatment involves replacing the missing hormone






35. Corn mash whiskey - is clear after distillation - the aging process while in an oak barrel for years - is responsible for the golden color and distinct taste of bourbon (or Jack Daniels Tennessee whiskey)






36. Is a lab technique used to separate various sized restriction fragments






37. Gene therapy involves insertion of particular recombinant DNA sequences that encode for ___________; used increasingly to replace missing or mutated genes to correct inherited defects






38. Enabling the large- scale production of life- saving or life- altering hormones and enzymes of human origin






39. Almost 6000 yrs ago in wine and bread making - involves practical application of microorganisms in manufacturing products or carrying out a particular decomposition - most involve the actions of bacteria - molds - yeasts adn algae that have been sele






40. Detecting variations in DNA sequence at same location in genome of different individuals;






41. Ethyl alcohol






42. Genetic Engineering is based on the ability to manipulate DNA by using a variety of ___________;allow DNA to cut precisely determined points along each strand - to be separated according to size of fragments and to be sequenced to determine exact com






43. Fermented and distilled corn mash - the state of Kentucky holds the patent on the word bourbon in reference to corn mash whiskey. Jack Daniels - produced in Tennessee is bourbon but NOT CALLED bourbon due to this patent






44. Involves removal of a selected gene from on organism (genetic donor) followed by its propagation in a different organism (cloning host)






45. Pieces of DNA produced by endonucleases - when in the laboratory - endonucleases are used to cleave DNA at desired sites - allowing them to be seperated by Gel Electrophoresis by size - then allowing the nucleotide sequences to be isolated and ident






46. Can be inserted into plants or animals to produce organisms that are better adapted to their environments - has been shown to cause improvements in crops by improving things such as drought resistance and has conveyed immunity to those consuming them






47. Is essential in the process of clot dissolution associated with myocardial and cerebral infarctions






48. To deliberately remove genetic material from one organism - and combine it with the genetic material of another






49. Are bacterial enzymes that recognize foreign DNA and are capable of breaking the bonds between adjacent nucleotides; each has a specific nucleotide sequence as its target






50. Actions of microbial enzymes that break down flour proteins ( gluten) and give dough its elasticity