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Test your basic knowledge |
Genetic Engineering Biotechnology
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Subject
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engineering
Instructions:
Answer 50 questions in 15 minutes.
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Match each statement with the correct term.
Don't refresh. All questions and answers are randomly picked and ordered every time you load a test.
This is a study tool. The 3 wrong answers for each question are randomly chosen from answers to other questions. So, you might find at times the answers obvious, but you will see it re-enforces your understanding as you take the test each time.
1. yeasts ferment carbs in fruits & grains anaerobically resulting in producing ethyl alcohol
Gene therapy involves
Hemophilia A
Inherent differences in what is used to identify humans - animals and microbes?
ethyl alcohol
2. Migration is due to attraction of opposite charges - smaller fragments move faster than larger ones - which allows the restriction fragments to be not just seperated but isolated and identified
Gene therapy involves
Plasmin
causes the restriction fragments to move through the gel during the process of gel electrophoresis
lab technique of Gel Electrophoresis
3. Gel electrophoresis is a process based on migration of negatively charged DNA fragments through a gel - toward a positive electrode
lab technique of Gel Electrophoresis
Baker's Yeast
used to replace missing or mutated genes to correct inherited defects
insulin
4. Although the availability of HGH was limited at best - demand exceeding the opportunity of supply - the potential transmission of infectious agents such as the prion associated with Creutzfedt- Jacob disease was life threatening to the individual rec
missing or dysfunctional proteins
the complication of most concern in treating Pituitary Dwarfism using human cadavers
Baker's Yeast
Genetic markers and unique DNA profiling can also be used by potential parents
5. Plasmids and bacteriophages
What can limit the treatment of diseases such as Type 1 Diabetes - Dwarfism and Hemophilia A?
Bourbon
most effective DNA vectors
Microbial Enzymes break down what?
6. Ethyl alcohol
Endonuclease enzymes
The first gene therapy patient
yeast + sugar => ethanol + carbon dioxide =
Gene therapy works because...
7. Involves manipulation of an organism's genome - Used in conjunction with biotechnology - which involves practical use of an organism's biochemical and metabolic pathways for the production of food products - chemicals - drugs andother products
Gel electrophoresis
Sour Dough
Genetic Engineering
Use of DNA sequencing procedures combined with amplification techniques such as polymerase chain reactions allow what?
8. Was a 4 yr old girl - in 1990 suffering from severe immunodeficiency disease caused by lack of enzyme adenosine deaminase (ADA). Researchers at the National Institutes of Health - transfused her own blood cells that had been engineered to contain the
Tissue Plasminogen Activator (tPA)
Genetic Engineering
The first gene therapy patient
Use of DNA sequencing procedures combined with amplification techniques such as polymerase chain reactions allow what?
9. Once the specific functional protein will be synthesized - the availbility of the protein will eliminate the disease condition
used to replace missing or mutated genes to correct inherited defects
yeast + sugar => ethanol + carbon dioxide =
Gene therapy works because...
causes uniqueness of DNA of an individual
10. Genetic Engineering is based on the ability to manipulate DNA by using a variety of ___________;allow DNA to cut precisely determined points along each strand - to be separated according to size of fragments and to be sequenced to determine exact com
Gene therapy involves
recombinant DNA techniques
Dwarfism
characteristics of a good cloning host
11. Identification of the gene responsible for the defective or absent protein - identification - isolation - cloning of the normal gene and transfection into the cells of the individual with the disease.
Gene therapy involves
Use of DNA sequencing procedures combined with amplification techniques such as polymerase chain reactions allow what?
Transfection of recombinant DNA
Gene therapy works because...
12. The actual sequence of nucleotides that make up the DNA molecule; we have due to lab techniques in genetic engineering - the ability to creat a DNA fingerprint ( DNA type or DNA profile) that is unique identifying characteristic of an individual
causes uniqueness of DNA of an individual
lactic acid
Bourbon
transgenic or genetically - modified organisms
13. Incompatible DNA of bacteriophages and plasmids
what endonuclease protects against In bacteria cells
recombinant DNA techniques
Transfection
plants or animals
14. A good recombinant vector must be capable of carrying a significant fragment of DNA and readily accept it
causes uniqueness of DNA of an individual
DNA profiles are established by...
Plasmin
requirements for a host to be a suitable vector for transfection
15. Limited availablity of the essential - missing or deficient hormone - which can be aided by pharmaceutical companies use of recombinant DNA technology.
nucleotide sequences
What can limit the treatment of diseases such as Type 1 Diabetes - Dwarfism and Hemophilia A?
Gene Therapy is the process in which
Dwarfism
16. Caused by a lack of adequate Human Growth Hormone (HGH); once treated by harvesting HGH from the pituitary glands of human cadavers
Dwarfism
characteristics of a good cloning host
benefit of restriction fragments being seperated through the process of gel electrophoresis
recombinant DNA techniques
17. Derived from potatos is the most widely marketed and consumed alcoholic beverage
vodka
Gene therapy
Type 1 Diabetes
Plasmin
18. A faulty gene in humans suffering from a disease which is due to the lack of a protein or the presence of a defective protein is corrected or reparied; inherent benefit is to permanently cure physiological dysfunction by repairing a genetic defect
Rum
Gene Therapy is the process in which
the complication of most concern in treating Pituitary Dwarfism using human cadavers
benefit of restriction fragments being seperated through the process of gel electrophoresis
19. Anaerobic fermentation in skeletal muscles produces
Modern applications of Biotechnology
lactic acid
Genetic Engineering
Gene therapy involves
20. Recombinant DNA may be inserted into ________ to produce organisms that are better adapted to their environments - may not be disease or drought tolerant - may provide more nutritional properties - may be easier to grow - or may even convey immunity
plants or animals
most effective DNA vectors
Inherent differences in what is used to identify humans - animals and microbes?
Recombinant DNA technology has changed the outcomes of many diseases and conditions by...
21. Enabling the large- scale production of life- saving or life- altering hormones and enzymes of human origin
Tissue Plasminogen Activator (tPA)
Recombinant DNA technology has changed the outcomes of many diseases and conditions by...
Inherent differences in what is used to identify humans - animals and microbes?
Pharmacuetical companies
22. Insertion of particular recombinant DNA sequences that encode missing or dysfunctional proteins
Biotechnology
Type 1 Diabetes
Gene therapy involves
Gene therapy
23. Final product of recombinant DNA may be production of a particular protein such as _______ - human growth factor and certain clotting factors; recombinant DNA is transferred into bacteria - which can utilize routine protein synthesis mechanisms to pr
Biotechnology originated___
Pharmacuetical companies
insulin
Endonuclease enzymes
24. Incorportates air into the dough
Tissue Plasminogen Activator (tPA)
kneading dough
Sour Dough
insulin
25. Pieces of DNA produced by endonucleases - when in the laboratory - endonucleases are used to cleave DNA at desired sites - allowing them to be seperated by Gel Electrophoresis by size - then allowing the nucleotide sequences to be isolated and ident
Restriction Fragments
Dwarfism
Tissue Plasminogen Activator (tPA)
Bourbon
26. Derived from sugar cane juice - is a primary alcoholic product of the caribbean islands - can be purchased in several different concentrations
Rum
characteristics of a good cloning host
Gene therapy works because...
skeletal muscles
27. Can revert to anaerobic fermentation in the event they have exhausted their oxygen supply - creating lactic acid
skeletal muscles
DNA profiles are established by...
missing or dysfunctional proteins
Sour Dough
28. Saccharomyctes cervisiae
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29. A lack of an essential hormone causes the disease and treatment involves replacing the missing hormone
Transfection of recombinant DNA
In diseases such as Type 1 Diabetes - Dwarfism - and Hemophilia A
Microbial Enzymes break down what?
skeletal muscles
30. Use of an organism's biochemical processes to produce a needed product - These processes have been greatly enhanced by use of recombinant DNA which allows organisms to be genetically modified to accomplish tasks that were previously not possible
Hemophilia A
Biotechnology
Genetic Engineering
most effective DNA vectors
31. Profile established by analysis of multiple markers provides unique characteristic of individual whose DNA is presented;
Genetic markers and unique DNA profiling can also be used by potential parents
DNA profiles are established by...
Biotechnology originated___
Rum
32. Actions of microbial enzymes that break down flour proteins ( gluten) and give dough its elasticity
Microbial Enzymes break down what?
Type 1 Diabetes
Gel electrophoresis
Gene therapy involves
33. Corn mash whiskey - is clear after distillation - the aging process while in an oak barrel for years - is responsible for the golden color and distinct taste of bourbon (or Jack Daniels Tennessee whiskey)
genetic probe testing
moon shine
In diseases such as Type 1 Diabetes - Dwarfism - and Hemophilia A
nucleotide sequences
34. Gene therapy involves insertion of particular recombinant DNA sequences that encode for ___________; used increasingly to replace missing or mutated genes to correct inherited defects
what endonuclease protects against In bacteria cells
missing or dysfunctional proteins
Pharmacuetical companies
lactic acid
35. Recombinant DNA may enable __________ to determine the likelihood of a praticular genetic disease long before the appearance of symptoms associated with the disease;early detection may allow intervention before the occurrence of irreversible damage.
Dwarfism
genetic probe testing
What DNA fingerprinting methods depend on?
vodka
36. Forensic medicin; examples : fingerprints - hair - skin - fingernails - semen - blood or saliva
the first uses of DNA fingerprinting
causes the restriction fragments to move through the gel during the process of gel electrophoresis
skeletal muscles
Biotechnology originated___
37. Use recombinant DNA technology to synthesize products that cannot be manufactured by other methods - or cannot be manufactured as efficiently or effectively by other methods
Modern applications of Biotechnology
The degree of uniquesness in DNA of an individual is directly proportional to ___
Pharmacuetical companies
Tissue Plasminogen Activator (tPA)
38. Which encodes for a particular protein product into the genome of appropriate host organism transforms the host organism into a production plant for the protein that is the genetic expression of a particular sequence - then - by means of routine bioc
Recombinant DNA technology has changed the outcomes of many diseases and conditions by...
Transfection of recombinant DNA
Genetic markers and unique DNA profiling can also be used by potential parents
benefit of restriction fragments being seperated through the process of gel electrophoresis
39. Is essential in the process of clot dissolution associated with myocardial and cerebral infarctions
Plasmin
what endonuclease protects against In bacteria cells
Pharmacuetical companies
In diseases such as Type 1 Diabetes - Dwarfism - and Hemophilia A
40. Are observable variations in DNA which help establish a profile
Genetic Markers
ethyl alcohol
Microbial Enzymes break down what?
Genetic Engineering
41. The process of introducing foreign genes into a cloning host organism
Transfection
Modern applications of Biotechnology
Recombinant DNA technology has changed the outcomes of many diseases and conditions by...
Biotechnology originated___
42. Used in industry - agriculture - food sciences and environmental protection. Some utilize genetic engineering techniques to achieve a desired outcome or rely on natu4ral biochemical and metabolic activities of organisms
Modern applications of Biotechnology
lactic acid
requirements for a host to be a suitable vector for transfection
DNA profiles are established by...
43. Lactobacillus Sanfrancisco
Sour Dough
what endonuclease protects against In bacteria cells
Baker's Yeast
lab technique of Gel Electrophoresis
44. Involves removal of a selected gene from on organism (genetic donor) followed by its propagation in a different organism (cloning host)
Gene therapy involves
Hemophilia A
In diseases such as Type 1 Diabetes - Dwarfism - and Hemophilia A
Cloning
45. Gene therapy
Genetic markers and unique DNA profiling can also be used by potential parents
Type 1 Diabetes
used to replace missing or mutated genes to correct inherited defects
Genetic Markers
46. Is a lab technique used to separate various sized restriction fragments
Use of DNA sequencing procedures combined with amplification techniques such as polymerase chain reactions allow what?
Gel electrophoresis
The first gene therapy patient
the complication of most concern in treating Pituitary Dwarfism using human cadavers
47. Detecting variations in DNA sequence at same location in genome of different individuals;
Gene therapy works because...
requirements for a host to be a suitable vector for transfection
What DNA fingerprinting methods depend on?
Sour Dough
48. Caused by a lack of adequate clotting factor VIII;
the complication of most concern in treating Pituitary Dwarfism using human cadavers
plants or animals
Hemophilia A
Rum
49. Inherent differences in ________ of DNA are used to identify humans - animals - and microbes;DNA analysis has revolutionized fields such as epidemiology and criminalistics; DNA technology is now common - place in forensic investigations
nucleotide sequences
kneading dough
characteristics of a good cloning host
advent of genetic engineering techniques has provided
50. Enzyme required for conversion of inactive protein plasminogen to its active form plasmin; is used to dissolve clots associated with myocardial and cerebral infarctions - availablity of tPA from human origin is limitied and not sufficient to meet the
Plasmin
most effective DNA vectors
Gene therapy works because...
Tissue Plasminogen Activator (tPA)
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