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Genetic Engineering Biotechnology

Subject : engineering
Instructions:
  • Answer 50 questions in 15 minutes.
  • If you are not ready to take this test, you can study here.
  • Match each statement with the correct term.
  • Don't refresh. All questions and answers are randomly picked and ordered every time you load a test.

This is a study tool. The 3 wrong answers for each question are randomly chosen from answers to other questions. So, you might find at times the answers obvious, but you will see it re-enforces your understanding as you take the test each time.
1. Fermented and distilled corn mash - the state of Kentucky holds the patent on the word bourbon in reference to corn mash whiskey. Jack Daniels - produced in Tennessee is bourbon but NOT CALLED bourbon due to this patent






2. Profile established by analysis of multiple markers provides unique characteristic of individual whose DNA is presented;






3. yeasts ferment carbs in fruits & grains anaerobically resulting in producing ethyl alcohol






4. After the actual size of individual fragments are determined - the nucleotide sequence which has been isolated and identified - can be inserted into an acceptable cloning host through the process of transfection






5. Derived from potatos is the most widely marketed and consumed alcoholic beverage






6. Is a lab technique used to separate various sized restriction fragments






7. Forensic medicin; examples : fingerprints - hair - skin - fingernails - semen - blood or saliva






8. Caused by a lack of adequate Human Growth Hormone (HGH); once treated by harvesting HGH from the pituitary glands of human cadavers






9. Limited availablity of the essential - missing or deficient hormone - which can be aided by pharmaceutical companies use of recombinant DNA technology.






10. Are recombinant organisms produced by insertion of recombinant DNA fragments






11. Lactobacillus Sanfrancisco






12. Can revert to anaerobic fermentation in the event they have exhausted their oxygen supply - creating lactic acid






13. Corn mash whiskey - is clear after distillation - the aging process while in an oak barrel for years - is responsible for the golden color and distinct taste of bourbon (or Jack Daniels Tennessee whiskey)






14. Recombinant DNA may be inserted into ________ to produce organisms that are better adapted to their environments - may not be disease or drought tolerant - may provide more nutritional properties - may be easier to grow - or may even convey immunity






15. Gene therapy involves insertion of particular recombinant DNA sequences that encode for ___________; used increasingly to replace missing or mutated genes to correct inherited defects






16. Use recombinant DNA technology to synthesize products that cannot be manufactured by other methods - or cannot be manufactured as efficiently or effectively by other methods






17. Inherent differences in ________ of DNA are used to identify humans - animals - and microbes;DNA analysis has revolutionized fields such as epidemiology and criminalistics; DNA technology is now common - place in forensic investigations






18. Are bacterial enzymes that recognize foreign DNA and are capable of breaking the bonds between adjacent nucleotides; each has a specific nucleotide sequence as its target






19. A faulty gene in humans suffering from a disease which is due to the lack of a protein or the presence of a defective protein is corrected or reparied; inherent benefit is to permanently cure physiological dysfunction by repairing a genetic defect






20. Detecting variations in DNA sequence at same location in genome of different individuals;






21. Which encodes for a particular protein product into the genome of appropriate host organism transforms the host organism into a production plant for the protein that is the genetic expression of a particular sequence - then - by means of routine bioc






22. Genetic Engineering is based on the ability to manipulate DNA by using a variety of ___________;allow DNA to cut precisely determined points along each strand - to be separated according to size of fragments and to be sequenced to determine exact com






23. Ethyl alcohol






24. Pieces of DNA produced by endonucleases - when in the laboratory - endonucleases are used to cleave DNA at desired sites - allowing them to be seperated by Gel Electrophoresis by size - then allowing the nucleotide sequences to be isolated and ident






25. Enzyme required for conversion of inactive protein plasminogen to its active form plasmin; is used to dissolve clots associated with myocardial and cerebral infarctions - availablity of tPA from human origin is limitied and not sufficient to meet the






26. Migration is due to attraction of opposite charges - smaller fragments move faster than larger ones - which allows the restriction fragments to be not just seperated but isolated and identified






27. The process of introducing foreign genes into a cloning host organism






28. Actions of microbial enzymes that break down flour proteins ( gluten) and give dough its elasticity






29. The ability to produce recombinant proteins in bacteria and yeasts reather than isolating protein from animals or humans






30. Plasmids and bacteriophages






31. Involves removal of a selected gene from on organism (genetic donor) followed by its propagation in a different organism (cloning host)






32. Final product of recombinant DNA may be production of a particular protein such as _______ - human growth factor and certain clotting factors; recombinant DNA is transferred into bacteria - which can utilize routine protein synthesis mechanisms to pr






33. Nucleotide sequences of DNA






34. Fast growth rate - ability to be grown in large quantities using ordinary culture methods - Nonpathogenic -Genome that is well- delineated (mapped) - capable of accepting plasmids or bacteriophage vectors - maintains foreign genes through multiple ge






35. A good recombinant vector must be capable of carrying a significant fragment of DNA and readily accept it






36. DNA fingerprinting of specimens that contain only minute amounts of DNA or even old specimens that may not be suitable for other test methods






37. Gel electrophoresis is a process based on migration of negatively charged DNA fragments through a gel - toward a positive electrode






38. Can be inserted into plants or animals to produce organisms that are better adapted to their environments - has been shown to cause improvements in crops by improving things such as drought resistance and has conveyed immunity to those consuming them






39. Recombinant DNA may enable __________ to determine the likelihood of a praticular genetic disease long before the appearance of symptoms associated with the disease;early detection may allow intervention before the occurrence of irreversible damage.






40. A lack of an essential hormone causes the disease and treatment involves replacing the missing hormone






41. Are observable variations in DNA which help establish a profile






42. Incorportates air into the dough






43. Was a 4 yr old girl - in 1990 suffering from severe immunodeficiency disease caused by lack of enzyme adenosine deaminase (ADA). Researchers at the National Institutes of Health - transfused her own blood cells that had been engineered to contain the






44. Enabling the large- scale production of life- saving or life- altering hormones and enzymes of human origin






45. Gene therapy






46. To deliberately remove genetic material from one organism - and combine it with the genetic material of another






47. Degree of uniqueness is directly proportional to the prevalence of particular markers in comparison population; use of multiple markers allow significant increase in actual uniqueness of profile established






48. Use of an organism's biochemical processes to produce a needed product - These processes have been greatly enhanced by use of recombinant DNA which allows organisms to be genetically modified to accomplish tasks that were previously not possible






49. Once the specific functional protein will be synthesized - the availbility of the protein will eliminate the disease condition






50. Caused by a lack of adequate clotting factor VIII;