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Genetic Engineering Technology

Subject : engineering
Instructions:
  • Answer 50 questions in 15 minutes.
  • If you are not ready to take this test, you can study here.
  • Match each statement with the correct term.
  • Don't refresh. All questions and answers are randomly picked and ordered every time you load a test.

This is a study tool. The 3 wrong answers for each question are randomly chosen from answers to other questions. So, you might find at times the answers obvious, but you will see it re-enforces your understanding as you take the test each time.
1. Humans are more likely to generate an unwanted immune response to the mouse version of the antibody than to the humanized version






2. VNTR's (variable number tandem repeats) and Subsets (STR short tandem repeats (CODIS))






3. IPS (induced pluripotent) - ESC (embryonic stem cells - and ASC (adult stem cells






4. It sequentially breaks down toxic compounds - eventually to water and CO2






5. Can't be used if bacteria does not have the same post- translational modifications as eukaryotes --- no glycosylation - Can use bacteria to produce eukaryotic proteins only if the protein does not require any post- translational modifications. (Or un






6. Done after treatment has been marketed and used - studied in various populations






7. Responsible for the control of pesticides - herbicides - etc






8. Treat DNA with restriction enzyme - Use agarose gel electrophoresis to separate pieces - Chemically treat/heat/denature DNA (allows for single stranded probes to bind)






9. Informed consent is required: must know the risks (not actually receiving the drug)






10. 2 -00-5 -000 base pairs






11. Responsible for drug - food items - and cosmetics






12. Antigens are foreign substances that stimulate an immune response






13. Pieces of DNA that can accept carry and replicate other pieces of DNA






14. Responsible for growing and farming practices






15. Cell consistency - Scaffold design and prep - Bioreactor design - Producing in the right size - Time of production - Clinical use - Regulatory approval - Ethical questions






16. Small group - 20-80 SAFETY - to establish dose range and side effects;






17. Each type of the enzyme cuts DNA at a specific nucleotide sequence - DNA cutting enzymes - Bind and cut at recognition sequences or restriction sites - Either create cohesive (sticky) ends or blunt ends






18. The nucleotide sequences are present in the initial mRNA transcript but are excised prior to translation






19. Centers for Disease Control and Prevention - protects public health and safety - provides information about health decisions and promotes health programs - works with disease - food- borne pathogens - occupational safety.






20. The site for DNA replication that allows the plasmids to replicate separately from the host cell's chromosome - component of cloning vector that allows it to replicate itself inside a bacterial cell






21. Focuses on certain absolute principles which should follow out of sense of duty and should dictate our actions






22. Advancement and regulation of agriculture - pests - plants and vet biologics - growing and farming practices






23. HESC - ASCs - AFS - iPS






24. Scaffolds - cells - cultivation


25. Genes allow for selection and identification of bacteria that has been modified (undergone recombination)






26. Genetically type biological tissue - identify matching pairs (that cause undesirable traits in offspring) - and can show these traits early in development - check for curly calf syndrome - water head - fawn calf syndrome - dwarfism - dsytocia - and c






27. Plasmids that are successfully engineered are white - If the gene is blue it is not effected - White colonies are inserted into the clone of DNA on Bgal site - Allows for detection of successful ligation (in vector- based gene cloning)






28. Component of the cloning vector that allows for the selection of those bacteria colonies that actually were transformed by the vector






29. Adult- dervied stem cells - Can differentiate but not as pluripotent as hESCs






30. Chemically - Mechanically - New ways to take from levis






31. Induced pluripotent stem cells - nuclear reprogrammed somatic cells






32. Tested on those with disease - 100-300 EFFICACY






33. Greatest good for the greatest number






34. Turn off specific genes






35. Composition of matter (chemical union) - Method - Article of Manufacture - Machine






36. Fusion of two protoplast cells from different species: hybrid plant






37. Human embryonic stem cells - from embryos left over or created - have the ability to undergo differentiation - pluripotent






38. Not obvious - Have a use - be novel






39. 1 -000-3 -000 (double- blind) - COMPARITIVE BENEFIT TO OTHER CURRENT DRUGS - confirm effectiveness - monitor side effects - compare to used treatments - and collect info -






40. Embryo twinning (split embyos in half) - cloned from adult DNA






41. A problem with gene therapy






42. Short stretched of DNA with single base pair changes (wildtype) - Short length allows distinguishing which allele is present - Allows the probe to stay attached to the target DNA - Perfectly matched will cause a signal






43. Prevent crops from freezing (ice-) - slow the breakdown of food (tomatoes) - create pesticides to stop infestations






44. Amniotic fluid- derived stem cells - Coaxed to become other cells






45. US Patent and Trademark Office - only one required by the constitution - oversees patenting






46. Protective cold- induced proteins from fish that live in very cold climates






47. The act that started the clean up of sites that had hazardous substances






48. Both for reproductive cloning and therapeutic cloning - treat leukemia: create white blood cell - sickle cell anemia: cord blood - form bone in skull: fat cells - repair heart cells - neuron creation






49. The target antigen (protein) for the vaccine is more easily selected and the vaccines can be generated much faster in microbes than in eggs






50. Allows for transcription of RNA (in vivo and in vitro) by RNA polymerase