Test your basic knowledge |

Genetic Engineering Technology

Subject : engineering
Instructions:
  • Answer 50 questions in 15 minutes.
  • If you are not ready to take this test, you can study here.
  • Match each statement with the correct term.
  • Don't refresh. All questions and answers are randomly picked and ordered every time you load a test.

This is a study tool. The 3 wrong answers for each question are randomly chosen from answers to other questions. So, you might find at times the answers obvious, but you will see it re-enforces your understanding as you take the test each time.
1. A problem with gene therapy






2. Each type of the enzyme cuts DNA at a specific nucleotide sequence - DNA cutting enzymes - Bind and cut at recognition sequences or restriction sites - Either create cohesive (sticky) ends or blunt ends






3. RFLP and PCR






4. Treat DNA with restriction enzyme - Use agarose gel electrophoresis to separate pieces - Chemically treat/heat/denature DNA (allows for single stranded probes to bind)






5. Responsible for growing and farming practices






6. It sequentially breaks down toxic compounds - eventually to water and CO2






7. Small group - 20-80 SAFETY - to establish dose range and side effects;






8. American Association for Laboratory Animal Science - oversees the treatment of laboratory animals -






9. US Patent and Trademark Office - only one required by the constitution - oversees patenting






10. Used in fingerprinting - Less DNA and can be slightly degraded - Extremely sensitive to contaminating DNA






11. VNTR's (variable number tandem repeats) and Subsets (STR short tandem repeats (CODIS))






12. Advancement and regulation of agriculture - pests - plants and vet biologics - growing and farming practices






13. HESC - ASCs - AFS - iPS






14. Laboratory technique uses a DNA polymerase from bacteria that grow in geysers - Allows for the exponential increase in the synthesis of specific segments of DNA






15. Induced pluripotent stem cells - nuclear reprogrammed somatic cells






16. Amniotic fluid- derived stem cells - Coaxed to become other cells






17. Fusion of two protoplast cells from different species: hybrid plant






18. Must be able to define its specific utility (must know exactly what it does... specifically) - show its substantial utility (real world use) - meet the credible utility requirement (be backed by sound science) - and not be similar to something else (






19. Stretch of DNA with recognition sequences for various types of restriction enzymes - In plasmid - Allows for range of DNA fragments to be cloned in the plasmid






20. Short reproductive cycles - similar physiology to humans - sufficient database of knowledge about the organisms genetics - don't have dorsal fins and rows of large sharp teeth - they produce a relatively large number of offspring






21. Find genes that are associated with a disease - Find unknown means of infection - Find new targets for drugs and vaccines






22. Only 1/100th of DNA differs from person to person






23. Done after treatment has been marketed and used - studied in various populations






24. 2 -00-5 -000 base pairs






25. Restriction fragment length polymorphism: used in fingerprinting - Requires larger amount of DNA - Can not degrade






26. Protective cold- induced proteins from fish that live in very cold climates






27. Embryo twinning (split embyos in half) - cloned from adult DNA






28. Adult- dervied stem cells - Can differentiate but not as pluripotent as hESCs






29. Focuses on certain absolute principles which should follow out of sense of duty and should dictate our actions






30. 1 -000-3 -000 (double- blind) - COMPARITIVE BENEFIT TO OTHER CURRENT DRUGS - confirm effectiveness - monitor side effects - compare to used treatments - and collect info -






31. Pieces of DNA that can accept carry and replicate other pieces of DNA






32. Cells and matrixes






33. Expensive - have short life spans (short telomeres) - cloned from previous animals age






34. First cloned and expressed in bacteria - was effective because it DOES NOT require glycosylation - and so did not need 'eukaryotic- specific' post- translational modifications






35. The act that started the clean up of sites that had hazardous substances






36. Scaffolds - cells - cultivation


37. Composition of matter (chemical union) - Method - Article of Manufacture - Machine






38. Plasmids that are successfully engineered are white - If the gene is blue it is not effected - White colonies are inserted into the clone of DNA on Bgal site - Allows for detection of successful ligation (in vector- based gene cloning)






39. Component of the cloning vector that allows for the selection of those bacteria colonies that actually were transformed by the vector






40. Isolate cells - inject gene - then reimplant cell






41. IPS (induced pluripotent) - ESC (embryonic stem cells - and ASC (adult stem cells






42. Soil bacterium that infects plants - contains TI plasmid that can be genetically modified - DNA from the TI plasmid integrates with DNA of the host cell






43. Not obvious - Have a use - be novel






44. Responsible for the control of pesticides - herbicides - etc






45. Informed consent is required: must know the risks (not actually receiving the drug)






46. Human embryonic stem cells - from embryos left over or created - have the ability to undergo differentiation - pluripotent






47. Both for reproductive cloning and therapeutic cloning - treat leukemia: create white blood cell - sickle cell anemia: cord blood - form bone in skull: fat cells - repair heart cells - neuron creation






48. Injection into the organism






49. 1-6 nucleotide repeats throughout the chromosome - 13 unique STRs used by the FBI for testing (CODIS)






50. Can't be used if bacteria does not have the same post- translational modifications as eukaryotes --- no glycosylation - Can use bacteria to produce eukaryotic proteins only if the protein does not require any post- translational modifications. (Or un