Test your basic knowledge |

Genetic Engineering Technology

Subject : engineering
Instructions:
  • Answer 50 questions in 15 minutes.
  • If you are not ready to take this test, you can study here.
  • Match each statement with the correct term.
  • Don't refresh. All questions and answers are randomly picked and ordered every time you load a test.

This is a study tool. The 3 wrong answers for each question are randomly chosen from answers to other questions. So, you might find at times the answers obvious, but you will see it re-enforces your understanding as you take the test each time.
1. Amniotic fluid- derived stem cells - Coaxed to become other cells






2. Adult- dervied stem cells - Can differentiate but not as pluripotent as hESCs






3. Retrovirus - mediated transgenics (infect mouse embryo with retrovirus and implant) - pronuclear microinjection (introduce transgenic DNA during zygote development - inject into sperm or egg) - embryonic stem cell method (embryonic stem cells mixed w






4. Human embryonic stem cells - from embryos left over or created - have the ability to undergo differentiation - pluripotent






5. 1-6 nucleotide repeats throughout the chromosome - 13 unique STRs used by the FBI for testing (CODIS)






6. Chemically - Mechanically - New ways to take from levis






7. Soil bacterium that infects plants - contains TI plasmid that can be genetically modified - DNA from the TI plasmid integrates with DNA of the host cell






8. Used in fingerprinting - Less DNA and can be slightly degraded - Extremely sensitive to contaminating DNA






9. 2 -00-5 -000 base pairs






10. Treat DNA with restriction enzyme - Use agarose gel electrophoresis to separate pieces - Chemically treat/heat/denature DNA (allows for single stranded probes to bind)






11. Short reproductive cycles - similar physiology to humans - sufficient database of knowledge about the organisms genetics - don't have dorsal fins and rows of large sharp teeth - they produce a relatively large number of offspring






12. Environmental Protection Agency - protecting endangered species to establishing emission standards for cars - regulates pesticides and herbicides - gmo's with pest resistance - monitors pesticide levels to ensure safe eating levels - the control of p






13. First cloned and expressed in bacteria - was effective because it DOES NOT require glycosylation - and so did not need 'eukaryotic- specific' post- translational modifications






14. Genes allow for selection and identification of bacteria that has been modified (undergone recombination)






15. IPS (induced pluripotent) - ESC (embryonic stem cells - and ASC (adult stem cells






16. Not obvious - Have a use - be novel






17. Scaffolds - cells - cultivation


18. Greatest good for the greatest number






19. Responsible for the standard of treatment for laboratory animals






20. Done after treatment has been marketed and used - studied in various populations






21. Antigens are foreign substances that stimulate an immune response






22. Stretch of DNA with recognition sequences for various types of restriction enzymes - In plasmid - Allows for range of DNA fragments to be cloned in the plasmid






23. Sequences that allows the nucleotide sequencing of cloned DNA fragments that are inserted into the plasmid






24. Isolate cells - inject gene - then reimplant cell






25. US Patent and Trademark Office - only one required by the constitution - oversees patenting






26. Focuses on certain absolute principles which should follow out of sense of duty and should dictate our actions






27. Responsible for the control of pesticides - herbicides - etc






28. VNTR's (variable number tandem repeats) and Subsets (STR short tandem repeats (CODIS))






29. Expensive - have short life spans (short telomeres) - cloned from previous animals age






30. The target antigen (protein) for the vaccine is more easily selected and the vaccines can be generated much faster in microbes than in eggs






31. Component of the cloning vector that allows for the selection of those bacteria colonies that actually were transformed by the vector






32. To create or modulate tissue to replace or augment diseased or damaged tissue






33. Small group - 20-80 SAFETY - to establish dose range and side effects;






34. Humans are more likely to generate an unwanted immune response to the mouse version of the antibody than to the humanized version






35. Restriction fragment length polymorphism: used in fingerprinting - Requires larger amount of DNA - Can not degrade






36. American Association for Laboratory Animal Science - oversees the treatment of laboratory animals -






37. Centers for Disease Control and Prevention - protects public health and safety - provides information about health decisions and promotes health programs - works with disease - food- borne pathogens - occupational safety.






38. It sequentially breaks down toxic compounds - eventually to water and CO2






39. Introduced as a sedative in 50's - caused birth defects - caused stricter regulations testing before drugs and pesticides were licensed






40. Advancement and regulation of agriculture - pests - plants and vet biologics - growing and farming practices






41. Genetically type biological tissue - identify matching pairs (that cause undesirable traits in offspring) - and can show these traits early in development - check for curly calf syndrome - water head - fawn calf syndrome - dwarfism - dsytocia - and c






42. 1 -000-3 -000 (double- blind) - COMPARITIVE BENEFIT TO OTHER CURRENT DRUGS - confirm effectiveness - monitor side effects - compare to used treatments - and collect info -






43. Animal and Plant Health Inspection Service - branch of USDA - protects agriculture from pest and diseases - permits for developing gmo's






44. The nucleotide sequences are present in the initial mRNA transcript but are excised prior to translation






45. Plasmids that are successfully engineered are white - If the gene is blue it is not effected - White colonies are inserted into the clone of DNA on Bgal site - Allows for detection of successful ligation (in vector- based gene cloning)






46. Must be able to define its specific utility (must know exactly what it does... specifically) - show its substantial utility (real world use) - meet the credible utility requirement (be backed by sound science) - and not be similar to something else (






47. Allows for transcription of RNA (in vivo and in vitro) by RNA polymerase






48. Skin and cartilage






49. Only 1/100th of DNA differs from person to person






50. Embryo twinning (split embyos in half) - cloned from adult DNA






?:?:)

Search BasicVersity Tests



OR

Browse BasicVersity