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Genetic Engineering Technology

Subject : engineering
Instructions:
  • Answer 50 questions in 15 minutes.
  • If you are not ready to take this test, you can study here.
  • Match each statement with the correct term.
  • Don't refresh. All questions and answers are randomly picked and ordered every time you load a test.

This is a study tool. The 3 wrong answers for each question are randomly chosen from answers to other questions. So, you might find at times the answers obvious, but you will see it re-enforces your understanding as you take the test each time.
1. VNTR's (variable number tandem repeats) and Subsets (STR short tandem repeats (CODIS))






2. Soil bacterium that infects plants - contains TI plasmid that can be genetically modified - DNA from the TI plasmid integrates with DNA of the host cell






3. HESC - ASCs - AFS - iPS






4. Responsible for drug - food items - and cosmetics






5. Humans are more likely to generate an unwanted immune response to the mouse version of the antibody than to the humanized version






6. Fusion of two protoplast cells from different species: hybrid plant






7. 1 -000-3 -000 (double- blind) - COMPARITIVE BENEFIT TO OTHER CURRENT DRUGS - confirm effectiveness - monitor side effects - compare to used treatments - and collect info -






8. Introduced as a sedative in 50's - caused birth defects - caused stricter regulations testing before drugs and pesticides were licensed






9. Responsible for the control of pesticides - herbicides - etc






10. Component of the cloning vector that allows for the selection of those bacteria colonies that actually were transformed by the vector






11. Chemically - Mechanically - New ways to take from levis






12. Stretch of DNA with recognition sequences for various types of restriction enzymes - In plasmid - Allows for range of DNA fragments to be cloned in the plasmid






13. Isolate cells - inject gene - then reimplant cell






14. Pieces of DNA that can accept carry and replicate other pieces of DNA






15. Environmental Protection Agency - protecting endangered species to establishing emission standards for cars - regulates pesticides and herbicides - gmo's with pest resistance - monitors pesticide levels to ensure safe eating levels - the control of p






16. Laboratory technique uses a DNA polymerase from bacteria that grow in geysers - Allows for the exponential increase in the synthesis of specific segments of DNA






17. Cell consistency - Scaffold design and prep - Bioreactor design - Producing in the right size - Time of production - Clinical use - Regulatory approval - Ethical questions






18. The site for DNA replication that allows the plasmids to replicate separately from the host cell's chromosome - component of cloning vector that allows it to replicate itself inside a bacterial cell






19. Genes allow for selection and identification of bacteria that has been modified (undergone recombination)






20. Animal and Plant Health Inspection Service - branch of USDA - protects agriculture from pest and diseases - permits for developing gmo's






21. The target antigen (protein) for the vaccine is more easily selected and the vaccines can be generated much faster in microbes than in eggs






22. Both for reproductive cloning and therapeutic cloning - treat leukemia: create white blood cell - sickle cell anemia: cord blood - form bone in skull: fat cells - repair heart cells - neuron creation






23. National Institute of Health - foremost medical research center - makes important health discoveries






24. Tested on those with disease - 100-300 EFFICACY






25. 1-6 nucleotide repeats throughout the chromosome - 13 unique STRs used by the FBI for testing (CODIS)






26. Food and Drug Administration - in charge of processed foods and medication - oversees the lab practices - phase testing of drugs - drug - food items - cosmetics






27. It sequentially breaks down toxic compounds - eventually to water and CO2






28. Genetically type biological tissue - identify matching pairs (that cause undesirable traits in offspring) - and can show these traits early in development - check for curly calf syndrome - water head - fawn calf syndrome - dwarfism - dsytocia - and c






29. Focuses on certain absolute principles which should follow out of sense of duty and should dictate our actions






30. Short stretched of DNA with single base pair changes (wildtype) - Short length allows distinguishing which allele is present - Allows the probe to stay attached to the target DNA - Perfectly matched will cause a signal






31. Informed consent is required: must know the risks (not actually receiving the drug)






32. Induced pluripotent stem cells - nuclear reprogrammed somatic cells






33. Embryo twinning (split embyos in half) - cloned from adult DNA






34. Expensive - have short life spans (short telomeres) - cloned from previous animals age






35. Small group - 20-80 SAFETY - to establish dose range and side effects;






36. Used in fingerprinting - Less DNA and can be slightly degraded - Extremely sensitive to contaminating DNA






37. Composition of matter (chemical union) - Method - Article of Manufacture - Machine






38. Protective cold- induced proteins from fish that live in very cold climates






39. The act that started the clean up of sites that had hazardous substances






40. Only 1/100th of DNA differs from person to person






41. Centers for Disease Control and Prevention - protects public health and safety - provides information about health decisions and promotes health programs - works with disease - food- borne pathogens - occupational safety.






42. RFLP and PCR






43. Sequences that allows the nucleotide sequencing of cloned DNA fragments that are inserted into the plasmid






44. Short reproductive cycles - similar physiology to humans - sufficient database of knowledge about the organisms genetics - don't have dorsal fins and rows of large sharp teeth - they produce a relatively large number of offspring






45. Responsible for growing and farming practices






46. Amniotic fluid- derived stem cells - Coaxed to become other cells






47. Recognize and bind to the antigen - Each individual cell has ONE antibody - First exposure to a foreign protein: B- cells cross - react to the protein - proliferate - and produce antibodies against the protein






48. Must be able to define its specific utility (must know exactly what it does... specifically) - show its substantial utility (real world use) - meet the credible utility requirement (be backed by sound science) - and not be similar to something else (






49. Restriction fragment length polymorphism: used in fingerprinting - Requires larger amount of DNA - Can not degrade






50. Is produced by a hybridoma (the fusion of a mature b- cell and an 'immortal' myeloma cancer cell - Is a single type of antibody that cross - reacts to only one target - one portion of the antigen against which it was made - Are generally much more sp