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Genetic Engineering Technology

Subject : engineering
Instructions:
  • Answer 50 questions in 15 minutes.
  • If you are not ready to take this test, you can study here.
  • Match each statement with the correct term.
  • Don't refresh. All questions and answers are randomly picked and ordered every time you load a test.

This is a study tool. The 3 wrong answers for each question are randomly chosen from answers to other questions. So, you might find at times the answers obvious, but you will see it re-enforces your understanding as you take the test each time.
1. Is produced by a hybridoma (the fusion of a mature b- cell and an 'immortal' myeloma cancer cell - Is a single type of antibody that cross - reacts to only one target - one portion of the antigen against which it was made - Are generally much more sp






2. American Association for Laboratory Animal Science - oversees the treatment of laboratory animals -






3. Restriction fragment length polymorphism: used in fingerprinting - Requires larger amount of DNA - Can not degrade






4. Informed consent is required: must know the risks (not actually receiving the drug)






5. IPS (induced pluripotent) - ESC (embryonic stem cells - and ASC (adult stem cells






6. Component of the cloning vector that allows for the selection of those bacteria colonies that actually were transformed by the vector






7. Scaffolds - cells - cultivation


8. Not obvious - Have a use - be novel






9. Environmental Protection Agency - protecting endangered species to establishing emission standards for cars - regulates pesticides and herbicides - gmo's with pest resistance - monitors pesticide levels to ensure safe eating levels - the control of p






10. Cells and matrixes






11. Both for reproductive cloning and therapeutic cloning - treat leukemia: create white blood cell - sickle cell anemia: cord blood - form bone in skull: fat cells - repair heart cells - neuron creation






12. Responsible for the control of pesticides - herbicides - etc






13. Chemically - Mechanically - New ways to take from levis






14. Recognize and bind to the antigen - Each individual cell has ONE antibody - First exposure to a foreign protein: B- cells cross - react to the protein - proliferate - and produce antibodies against the protein






15. Fusion of two protoplast cells from different species: hybrid plant






16. Soil bacterium that infects plants - contains TI plasmid that can be genetically modified - DNA from the TI plasmid integrates with DNA of the host cell






17. Induced pluripotent stem cells - nuclear reprogrammed somatic cells






18. Can't be used if bacteria does not have the same post- translational modifications as eukaryotes --- no glycosylation - Can use bacteria to produce eukaryotic proteins only if the protein does not require any post- translational modifications. (Or un






19. Used in fingerprinting - Less DNA and can be slightly degraded - Extremely sensitive to contaminating DNA






20. Embryo twinning (split embyos in half) - cloned from adult DNA






21. Plasmids that are successfully engineered are white - If the gene is blue it is not effected - White colonies are inserted into the clone of DNA on Bgal site - Allows for detection of successful ligation (in vector- based gene cloning)






22. Isolate cells - inject gene - then reimplant cell






23. The site for DNA replication that allows the plasmids to replicate separately from the host cell's chromosome - component of cloning vector that allows it to replicate itself inside a bacterial cell






24. Animal and Plant Health Inspection Service - branch of USDA - protects agriculture from pest and diseases - permits for developing gmo's






25. Humans are more likely to generate an unwanted immune response to the mouse version of the antibody than to the humanized version






26. Must be able to define its specific utility (must know exactly what it does... specifically) - show its substantial utility (real world use) - meet the credible utility requirement (be backed by sound science) - and not be similar to something else (






27. Done after treatment has been marketed and used - studied in various populations






28. Responsible for drug - food items - and cosmetics






29. The act that started the clean up of sites that had hazardous substances






30. Cut small discs of leaf - culture in genetically modified agrobacter - Leaf discs are treated with hormones






31. To create or modulate tissue to replace or augment diseased or damaged tissue






32. Allows for transcription of RNA (in vivo and in vitro) by RNA polymerase






33. Tested on those with disease - 100-300 EFFICACY






34. First cloned and expressed in bacteria - was effective because it DOES NOT require glycosylation - and so did not need 'eukaryotic- specific' post- translational modifications






35. Only 1/100th of DNA differs from person to person






36. Composition of matter (chemical union) - Method - Article of Manufacture - Machine






37. Treat DNA with restriction enzyme - Use agarose gel electrophoresis to separate pieces - Chemically treat/heat/denature DNA (allows for single stranded probes to bind)






38. Injection into the organism






39. HESC - ASCs - AFS - iPS






40. 2 -00-5 -000 base pairs






41. Focuses on certain absolute principles which should follow out of sense of duty and should dictate our actions






42. Amniotic fluid- derived stem cells - Coaxed to become other cells






43. Cell consistency - Scaffold design and prep - Bioreactor design - Producing in the right size - Time of production - Clinical use - Regulatory approval - Ethical questions






44. A problem with gene therapy






45. Stretch of DNA with recognition sequences for various types of restriction enzymes - In plasmid - Allows for range of DNA fragments to be cloned in the plasmid






46. Find genes that are associated with a disease - Find unknown means of infection - Find new targets for drugs and vaccines






47. Short reproductive cycles - similar physiology to humans - sufficient database of knowledge about the organisms genetics - don't have dorsal fins and rows of large sharp teeth - they produce a relatively large number of offspring






48. Responsible for the standard of treatment for laboratory animals






49. Protective cold- induced proteins from fish that live in very cold climates






50. Sequences that allows the nucleotide sequencing of cloned DNA fragments that are inserted into the plasmid