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Genetic Engineering Technology

Subject : engineering
Instructions:
  • Answer 50 questions in 15 minutes.
  • If you are not ready to take this test, you can study here.
  • Match each statement with the correct term.
  • Don't refresh. All questions and answers are randomly picked and ordered every time you load a test.

This is a study tool. The 3 wrong answers for each question are randomly chosen from answers to other questions. So, you might find at times the answers obvious, but you will see it re-enforces your understanding as you take the test each time.
1. Fusion of two protoplast cells from different species: hybrid plant






2. Stretch of DNA with recognition sequences for various types of restriction enzymes - In plasmid - Allows for range of DNA fragments to be cloned in the plasmid






3. IPS (induced pluripotent) - ESC (embryonic stem cells - and ASC (adult stem cells






4. Greatest good for the greatest number






5. The nucleotide sequences are present in the initial mRNA transcript but are excised prior to translation






6. Can't be used if bacteria does not have the same post- translational modifications as eukaryotes --- no glycosylation - Can use bacteria to produce eukaryotic proteins only if the protein does not require any post- translational modifications. (Or un






7. 1-6 nucleotide repeats throughout the chromosome - 13 unique STRs used by the FBI for testing (CODIS)






8. Genetically type biological tissue - identify matching pairs (that cause undesirable traits in offspring) - and can show these traits early in development - check for curly calf syndrome - water head - fawn calf syndrome - dwarfism - dsytocia - and c






9. Laboratory technique uses a DNA polymerase from bacteria that grow in geysers - Allows for the exponential increase in the synthesis of specific segments of DNA






10. Done after treatment has been marketed and used - studied in various populations






11. Must be able to define its specific utility (must know exactly what it does... specifically) - show its substantial utility (real world use) - meet the credible utility requirement (be backed by sound science) - and not be similar to something else (






12. Not obvious - Have a use - be novel






13. Humans are more likely to generate an unwanted immune response to the mouse version of the antibody than to the humanized version






14. DNA copy of an mRNA molecule expressed in a cell






15. Introduced as a sedative in 50's - caused birth defects - caused stricter regulations testing before drugs and pesticides were licensed






16. Small group - 20-80 SAFETY - to establish dose range and side effects;






17. Responsible for the control of pesticides - herbicides - etc






18. Adult- dervied stem cells - Can differentiate but not as pluripotent as hESCs






19. Responsible for growing and farming practices






20. Responsible for the standard of treatment for laboratory animals






21. Amniotic fluid- derived stem cells - Coaxed to become other cells






22. HESC - ASCs - AFS - iPS






23. It sequentially breaks down toxic compounds - eventually to water and CO2






24. Responsible for drug - food items - and cosmetics






25. Informed consent is required: must know the risks (not actually receiving the drug)






26. The target antigen (protein) for the vaccine is more easily selected and the vaccines can be generated much faster in microbes than in eggs






27. Cut small discs of leaf - culture in genetically modified agrobacter - Leaf discs are treated with hormones






28. The act that started the clean up of sites that had hazardous substances






29. Each type of the enzyme cuts DNA at a specific nucleotide sequence - DNA cutting enzymes - Bind and cut at recognition sequences or restriction sites - Either create cohesive (sticky) ends or blunt ends






30. Restriction fragment length polymorphism: used in fingerprinting - Requires larger amount of DNA - Can not degrade






31. Food and Drug Administration - in charge of processed foods and medication - oversees the lab practices - phase testing of drugs - drug - food items - cosmetics






32. Short reproductive cycles - similar physiology to humans - sufficient database of knowledge about the organisms genetics - don't have dorsal fins and rows of large sharp teeth - they produce a relatively large number of offspring






33. A problem with gene therapy






34. Component of the cloning vector that allows for the selection of those bacteria colonies that actually were transformed by the vector






35. 1 -000-3 -000 (double- blind) - COMPARITIVE BENEFIT TO OTHER CURRENT DRUGS - confirm effectiveness - monitor side effects - compare to used treatments - and collect info -






36. Animal and Plant Health Inspection Service - branch of USDA - protects agriculture from pest and diseases - permits for developing gmo's






37. Soil bacterium that infects plants - contains TI plasmid that can be genetically modified - DNA from the TI plasmid integrates with DNA of the host cell






38. Advancement and regulation of agriculture - pests - plants and vet biologics - growing and farming practices






39. Cell consistency - Scaffold design and prep - Bioreactor design - Producing in the right size - Time of production - Clinical use - Regulatory approval - Ethical questions






40. 2 -00-5 -000 base pairs






41. To create or modulate tissue to replace or augment diseased or damaged tissue






42. VNTR's (variable number tandem repeats) and Subsets (STR short tandem repeats (CODIS))






43. Only 1/100th of DNA differs from person to person






44. Pieces of DNA that can accept carry and replicate other pieces of DNA






45. American Association for Laboratory Animal Science - oversees the treatment of laboratory animals -






46. Expensive - have short life spans (short telomeres) - cloned from previous animals age






47. Isolate cells - inject gene - then reimplant cell






48. Allows for transcription of RNA (in vivo and in vitro) by RNA polymerase






49. Composition of matter (chemical union) - Method - Article of Manufacture - Machine






50. Induced pluripotent stem cells - nuclear reprogrammed somatic cells