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Test your basic knowledge |
Genetic Engineering Technology
Start Test
Study First
Subject
:
engineering
Instructions:
Answer 50 questions in 15 minutes.
If you are not ready to take this test, you can
study here
.
Match each statement with the correct term.
Don't refresh. All questions and answers are randomly picked and ordered every time you load a test.
This is a study tool. The 3 wrong answers for each question are randomly chosen from answers to other questions. So, you might find at times the answers obvious, but you will see it re-enforces your understanding as you take the test each time.
1. Not obvious - Have a use - be novel
AALAS
What must an invention do in order to be patentable
Human insulin
Antibodies
2. First cloned and expressed in bacteria - was effective because it DOES NOT require glycosylation - and so did not need 'eukaryotic- specific' post- translational modifications
Phase III
Was to purify DNA
Human insulin
EPA
3. Plasmids that are successfully engineered are white - If the gene is blue it is not effected - White colonies are inserted into the clone of DNA on Bgal site - Allows for detection of successful ligation (in vector- based gene cloning)
Ways to clone animals
AALAS
Reasons for studying microbes genomes
blue- white (B- galactosidase)
4. Focuses on certain absolute principles which should follow out of sense of duty and should dictate our actions
Deontological (Kantian)
Human insulin
NIH
Phase IV
5. The nucleotide sequences are present in the initial mRNA transcript but are excised prior to translation
Reasons for studying microbes genomes
PCR (polymerase chain reaction)
Intron
leaf fragment technique
6. 1-6 nucleotide repeats throughout the chromosome - 13 unique STRs used by the FBI for testing (CODIS)
What are the major types of patents
Vector
Detecting microsatellites (STR)
What must an invention do in order to be patentable
7. Only 1/100th of DNA differs from person to person
Restriction Enzymes
the expected advantages of producing recombinant vaccines in microbes instead of eggs are...
Reasons why you can not just compare DNA in fingerprinting
give three examples of how genetically engineered plants can increase food production
8. Must be able to define its specific utility (must know exactly what it does... specifically) - show its substantial utility (real world use) - meet the credible utility requirement (be backed by sound science) - and not be similar to something else (
Selectable marker genes
USDA
How patents for DNA sequences are different
Reasons why you can not just compare DNA in fingerprinting
9. US Patent and Trademark Office - only one required by the constitution - oversees patenting
AALAS
Was to purify DNA
USPto...
Phase III
10. Amniotic fluid- derived stem cells - Coaxed to become other cells
EPA
Phase III
Ex vivo gene therapy
AFS
11. Fusion of two protoplast cells from different species: hybrid plant
How patents for DNA sequences are different
protoplast fusion
Ex vivo gene therapy
the expected advantages of producing recombinant vaccines in microbes instead of eggs are...
12. IPS (induced pluripotent) - ESC (embryonic stem cells - and ASC (adult stem cells
APHis...
Utilitarian
In vivo gene therapy
three types of stem cells
13. Animal and Plant Health Inspection Service - branch of USDA - protects agriculture from pest and diseases - permits for developing gmo's
Tissue regeneration's 3 main areas
APHis...
How can bacteria be used humans
The general importance of Thalidomide
14. Introduced as a sedative in 50's - caused birth defects - caused stricter regulations testing before drugs and pesticides were licensed
Reasons why you can not just compare DNA in fingerprinting
The general importance of Thalidomide
leaf fragment technique
Two types of forensic DNA testing
15. Responsible for the standard of treatment for laboratory animals
iPS
AALAS
Reasons for studying microbes genomes
Detecting microsatellites (STR)
16. Food and Drug Administration - in charge of processed foods and medication - oversees the lab practices - phase testing of drugs - drug - food items - cosmetics
evaluated in DNA fingerprinting
Antibodies
FDA
Problems to overcome in tissue regeneration
17. RFLP and PCR
Two types of forensic DNA testing
DNA sequencing primers
B- cells
the expected advantages of producing recombinant vaccines in microbes instead of eggs are...
18. Cell consistency - Scaffold design and prep - Bioreactor design - Producing in the right size - Time of production - Clinical use - Regulatory approval - Ethical questions
Problems to overcome in tissue regeneration
Ethical dilemma using placebos - when they were on drugs before- how does FDA address this...
Tumors
Was to purify DNA
19. Stretch of DNA with recognition sequences for various types of restriction enzymes - In plasmid - Allows for range of DNA fragments to be cloned in the plasmid
EPA
Multiple cloning sites
Ways to introduce new genetic material into animals
Use of antisense RNA technology
20. Composition of matter (chemical union) - Method - Article of Manufacture - Machine
What are the major types of patents
A monoclonal antibody
Selectable marker genes
Antibodies
21. Environmental Protection Agency - protecting endangered species to establishing emission standards for cars - regulates pesticides and herbicides - gmo's with pest resistance - monitors pesticide levels to ensure safe eating levels - the control of p
Ways to clone animals
PCR (polymerase chain reaction)
FDA
EPA
22. Both for reproductive cloning and therapeutic cloning - treat leukemia: create white blood cell - sickle cell anemia: cord blood - form bone in skull: fat cells - repair heart cells - neuron creation
Ways to clone animals
iPS
Ex vivo gene therapy
uses of stem cells
23. Adult- dervied stem cells - Can differentiate but not as pluripotent as hESCs
Reasons for studying microbes genomes
ASCs
hESC
DNA cloning vectors size
24. HESC - ASCs - AFS - iPS
EPA
Was to purify DNA
Kinds of stem cells
A monoclonal antibody
25. Small group - 20-80 SAFETY - to establish dose range and side effects;
A monoclonal antibody
Use of SNPS
RNA polymerase promoter sequences
Phase I
26. Injection into the organism
The general importance of Thalidomide
A monoclonal antibody
Was to purify DNA
In vivo gene therapy
27. Chemically - Mechanically - New ways to take from levis
Tissue regeneration's 3 main areas
EPA
Was to purify DNA
Vector
28. The act that started the clean up of sites that had hazardous substances
blue- white (B- galactosidase)
Phase II
Superfund site
Ethical dilemma using placebos - when they were on drugs before- how does FDA address this...
29. To create or modulate tissue to replace or augment diseased or damaged tissue
B- cells
Examples of successful commercial tissue regeneration
Aim of tissue regeneration
Origin of replication
30. Embryo twinning (split embyos in half) - cloned from adult DNA
Origin of replication
FDA
Ways to clone animals
DNA cloning vectors size
31. Greatest good for the greatest number
Phase II
Phase I
Utilitarian
Was to purify DNA
32. Genetically type biological tissue - identify matching pairs (that cause undesirable traits in offspring) - and can show these traits early in development - check for curly calf syndrome - water head - fawn calf syndrome - dwarfism - dsytocia - and c
Restriction Enzymes
Use of SNPS
Phase I
AALAS
33. Scaffolds - cells - cultivation
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34. Protective cold- induced proteins from fish that live in very cold climates
anti- freeze proteins
Problems to overcome in tissue regeneration
DNA cloning vectors size
FDA
35. Antigens are foreign substances that stimulate an immune response
Antibodies
Restriction Enzymes
Drug resistance gene (amp)
Intron
36. Expensive - have short life spans (short telomeres) - cloned from previous animals age
Tumors
Why the cloning of animals is inefficient
Phase IV
What are the major types of patents
37. Advancement and regulation of agriculture - pests - plants and vet biologics - growing and farming practices
USDA
hESC
The general importance of Thalidomide
Vector
38. Short stretched of DNA with single base pair changes (wildtype) - Short length allows distinguishing which allele is present - Allows the probe to stay attached to the target DNA - Perfectly matched will cause a signal
give three examples of how genetically engineered plants can increase food production
Restriction Enzymes
Single Nucleotide Polymorphisms
Ways to introduce new genetic material into animals
39. A problem with gene therapy
B- cells
Desired traits for research animals
Tumors
three types of stem cells
40. Induced pluripotent stem cells - nuclear reprogrammed somatic cells
Phase IV
anti- freeze proteins
Kinds of stem cells
iPS
41. Isolate cells - inject gene - then reimplant cell
Tissue regeneration's 3 main areas
Ex vivo gene therapy
Utilitarian
Restriction Enzymes
42. It sequentially breaks down toxic compounds - eventually to water and CO2
Oxidation is important in bioremediation because...
Phase II
DNA sequencing primers
ASCs
43. Done after treatment has been marketed and used - studied in various populations
Intron
Phase IV
Why the cloning of animals is inefficient
Oxidation is important in bioremediation because...
44. DNA copy of an mRNA molecule expressed in a cell
Detecting microsatellites (STR)
blue- white (B- galactosidase)
cDNA
What must an invention do in order to be patentable
45. Humans are more likely to generate an unwanted immune response to the mouse version of the antibody than to the humanized version
What must an invention do in order to be patentable
B- cells
Aim of tissue regeneration
portions of mouse antibody genes that are used to make therapeutic mononclonal antibodies are routinely replaced with the equivalent portion from a human antibody gene because...
46. Short reproductive cycles - similar physiology to humans - sufficient database of knowledge about the organisms genetics - don't have dorsal fins and rows of large sharp teeth - they produce a relatively large number of offspring
leaf fragment technique
blue- white (B- galactosidase)
B- cells
Desired traits for research animals
47. Tested on those with disease - 100-300 EFFICACY
Phase II
Intron
evaluated in DNA fingerprinting
anti- freeze proteins
48. Used in fingerprinting - Less DNA and can be slightly degraded - Extremely sensitive to contaminating DNA
How patents for DNA sequences are different
RFLP analysis...
Single Nucleotide Polymorphisms
PCR (polymerase chain reaction)
49. Cut small discs of leaf - culture in genetically modified agrobacter - Leaf discs are treated with hormones
uses of stem cells
Drug resistance gene (amp)
Human insulin
leaf fragment technique
50. Responsible for the control of pesticides - herbicides - etc
RFLP
AALAS
The general importance of Thalidomide
EPA