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Test your basic knowledge |
Genetic Engineering Technology
Start Test
Study First
Subject
:
engineering
Instructions:
Answer 50 questions in 15 minutes.
If you are not ready to take this test, you can
study here
.
Match each statement with the correct term.
Don't refresh. All questions and answers are randomly picked and ordered every time you load a test.
This is a study tool. The 3 wrong answers for each question are randomly chosen from answers to other questions. So, you might find at times the answers obvious, but you will see it re-enforces your understanding as you take the test each time.
1. 2 -00-5 -000 base pairs
DNA cloning vectors size
Phase I
What tissue is made of
Why the cloning of animals is inefficient
2. Expensive - have short life spans (short telomeres) - cloned from previous animals age
Was to purify DNA
Why the cloning of animals is inefficient
FDA
AALAS
3. RFLP and PCR
Why the cloning of animals is inefficient
Two types of forensic DNA testing
PCR (polymerase chain reaction)
Origin of replication
4. Induced pluripotent stem cells - nuclear reprogrammed somatic cells
What tissue is made of
iPS
AFS
RNA polymerase promoter sequences
5. Can't be used if bacteria does not have the same post- translational modifications as eukaryotes --- no glycosylation - Can use bacteria to produce eukaryotic proteins only if the protein does not require any post- translational modifications. (Or un
Drug resistance gene (amp)
USDA
How can bacteria be used humans
Vector
6. Centers for Disease Control and Prevention - protects public health and safety - provides information about health decisions and promotes health programs - works with disease - food- borne pathogens - occupational safety.
Tissue regeneration's 3 main areas
The general importance of Thalidomide
USDA
CDC
7. Scaffolds - cells - cultivation
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8. To create or modulate tissue to replace or augment diseased or damaged tissue
protoplast fusion
Ways to clone animals
Aim of tissue regeneration
CDC
9. Antigens are foreign substances that stimulate an immune response
Antibodies
RFLP analysis...
NIH
Aim of tissue regeneration
10. The nucleotide sequences are present in the initial mRNA transcript but are excised prior to translation
Intron
Aim of tissue regeneration
anti- freeze proteins
AFS
11. Laboratory technique uses a DNA polymerase from bacteria that grow in geysers - Allows for the exponential increase in the synthesis of specific segments of DNA
APHis...
The general importance of Thalidomide
PCR
RFLP
12. Responsible for the control of pesticides - herbicides - etc
Multiple cloning sites
EPA
The general importance of Thalidomide
APHis...
13. Only 1/100th of DNA differs from person to person
Ways to introduce new genetic material into animals
Phase III
Reasons why you can not just compare DNA in fingerprinting
B- cells
14. Is produced by a hybridoma (the fusion of a mature b- cell and an 'immortal' myeloma cancer cell - Is a single type of antibody that cross - reacts to only one target - one portion of the antigen against which it was made - Are generally much more sp
APHis...
Selectable marker genes
the expected advantages of producing recombinant vaccines in microbes instead of eggs are...
A monoclonal antibody
15. Cell consistency - Scaffold design and prep - Bioreactor design - Producing in the right size - Time of production - Clinical use - Regulatory approval - Ethical questions
the expected advantages of producing recombinant vaccines in microbes instead of eggs are...
FDA
anti- freeze proteins
Problems to overcome in tissue regeneration
16. The target antigen (protein) for the vaccine is more easily selected and the vaccines can be generated much faster in microbes than in eggs
the expected advantages of producing recombinant vaccines in microbes instead of eggs are...
Vector
AFS
Phase IV
17. Food and Drug Administration - in charge of processed foods and medication - oversees the lab practices - phase testing of drugs - drug - food items - cosmetics
Kinds of stem cells
What tissue is made of
FDA
PCR (polymerase chain reaction)
18. Plasmids that are successfully engineered are white - If the gene is blue it is not effected - White colonies are inserted into the clone of DNA on Bgal site - Allows for detection of successful ligation (in vector- based gene cloning)
blue- white (B- galactosidase)
AALAS
Reasons for studying microbes genomes
RNA polymerase promoter sequences
19. Embryo twinning (split embyos in half) - cloned from adult DNA
Ethical dilemma using placebos - when they were on drugs before- how does FDA address this...
ASCs
Ways to clone animals
FDA
20. National Institute of Health - foremost medical research center - makes important health discoveries
How can bacteria be used humans
FDA
A monoclonal antibody
NIH
21. Done after treatment has been marketed and used - studied in various populations
Use of antisense RNA technology
B- cells
Phase IV
CDC
22. Genes allow for selection and identification of bacteria that has been modified (undergone recombination)
Single Nucleotide Polymorphisms
RFLP analysis...
Reasons for studying microbes genomes
Selectable marker genes
23. Must be able to define its specific utility (must know exactly what it does... specifically) - show its substantial utility (real world use) - meet the credible utility requirement (be backed by sound science) - and not be similar to something else (
How can bacteria be used humans
agrobacter characteristics
How patents for DNA sequences are different
B- cells
24. A problem with gene therapy
Tumors
USDA
Human insulin
A monoclonal antibody
25. Short reproductive cycles - similar physiology to humans - sufficient database of knowledge about the organisms genetics - don't have dorsal fins and rows of large sharp teeth - they produce a relatively large number of offspring
anti- freeze proteins
Examples of successful commercial tissue regeneration
Desired traits for research animals
USDA
26. Greatest good for the greatest number
uses of stem cells
Utilitarian
Single Nucleotide Polymorphisms
Use of antisense RNA technology
27. It sequentially breaks down toxic compounds - eventually to water and CO2
ASCs
FDA
Oxidation is important in bioremediation because...
In vivo gene therapy
28. Responsible for growing and farming practices
USDA
NIH
agrobacter characteristics
Examples of successful commercial tissue regeneration
29. Not obvious - Have a use - be novel
What must an invention do in order to be patentable
USPto...
Detecting microsatellites (STR)
Deontological (Kantian)
30. Advancement and regulation of agriculture - pests - plants and vet biologics - growing and farming practices
EPA
Phase III
In vivo gene therapy
USDA
31. Tested on those with disease - 100-300 EFFICACY
RNA polymerase promoter sequences
give three examples of how genetically engineered plants can increase food production
Phase II
FDA
32. DNA copy of an mRNA molecule expressed in a cell
USDA
The general importance of Thalidomide
cDNA
PCR
33. Short stretched of DNA with single base pair changes (wildtype) - Short length allows distinguishing which allele is present - Allows the probe to stay attached to the target DNA - Perfectly matched will cause a signal
Reasons why you can not just compare DNA in fingerprinting
USPto...
Single Nucleotide Polymorphisms
Ethical dilemma using placebos - when they were on drugs before- how does FDA address this...
34. US Patent and Trademark Office - only one required by the constitution - oversees patenting
ASCs
Ex vivo gene therapy
Multiple cloning sites
USPto...
35. Pieces of DNA that can accept carry and replicate other pieces of DNA
Vector
Drug resistance gene (amp)
Restriction Enzymes
leaf fragment technique
36. Injection into the organism
Examples of successful commercial tissue regeneration
In vivo gene therapy
DNA sequencing primers
Tissue regeneration's 3 main areas
37. Genetically type biological tissue - identify matching pairs (that cause undesirable traits in offspring) - and can show these traits early in development - check for curly calf syndrome - water head - fawn calf syndrome - dwarfism - dsytocia - and c
Origin of replication
FDA
Use of SNPS
AFS
38. Human embryonic stem cells - from embryos left over or created - have the ability to undergo differentiation - pluripotent
cDNA
EPA
hESC
DNA sequencing primers
39. Protective cold- induced proteins from fish that live in very cold climates
Drug resistance gene (amp)
CDC
three types of stem cells
anti- freeze proteins
40. Used in fingerprinting - Less DNA and can be slightly degraded - Extremely sensitive to contaminating DNA
PCR (polymerase chain reaction)
DNA sequencing primers
Tissue regeneration's 3 main areas
USDA
41. Composition of matter (chemical union) - Method - Article of Manufacture - Machine
What are the major types of patents
In vivo gene therapy
A monoclonal antibody
PCR (polymerase chain reaction)
42. Responsible for drug - food items - and cosmetics
three types of stem cells
iPS
the expected advantages of producing recombinant vaccines in microbes instead of eggs are...
FDA
43. The act that started the clean up of sites that had hazardous substances
Ex vivo gene therapy
Superfund site
APHis...
Multiple cloning sites
44. Cut small discs of leaf - culture in genetically modified agrobacter - Leaf discs are treated with hormones
Reasons why you can not just compare DNA in fingerprinting
APHis...
Restriction Enzymes
leaf fragment technique
45. Chemically - Mechanically - New ways to take from levis
Ethical dilemma using placebos - when they were on drugs before- how does FDA address this...
Restriction Enzymes
Was to purify DNA
FDA
46. Prevent crops from freezing (ice-) - slow the breakdown of food (tomatoes) - create pesticides to stop infestations
cDNA
RFLP analysis...
How patents for DNA sequences are different
give three examples of how genetically engineered plants can increase food production
47. Recognize and bind to the antigen - Each individual cell has ONE antibody - First exposure to a foreign protein: B- cells cross - react to the protein - proliferate - and produce antibodies against the protein
DNA sequencing primers
Phase I
Desired traits for research animals
B- cells
48. Skin and cartilage
DNA cloning vectors size
Examples of successful commercial tissue regeneration
Phase IV
evaluated in DNA fingerprinting
49. Informed consent is required: must know the risks (not actually receiving the drug)
Phase IV
Ethical dilemma using placebos - when they were on drugs before- how does FDA address this...
Detecting microsatellites (STR)
What tissue is made of
50. Responsible for the standard of treatment for laboratory animals
Tumors
AALAS
Utilitarian
APHis...