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Genetic Engineering Technology

Subject : engineering
Instructions:
  • Answer 50 questions in 15 minutes.
  • If you are not ready to take this test, you can study here.
  • Match each statement with the correct term.
  • Don't refresh. All questions and answers are randomly picked and ordered every time you load a test.

This is a study tool. The 3 wrong answers for each question are randomly chosen from answers to other questions. So, you might find at times the answers obvious, but you will see it re-enforces your understanding as you take the test each time.
1. Responsible for drug - food items - and cosmetics






2. Informed consent is required: must know the risks (not actually receiving the drug)






3. Restriction fragment length polymorphism: used in fingerprinting - Requires larger amount of DNA - Can not degrade






4. Prevent crops from freezing (ice-) - slow the breakdown of food (tomatoes) - create pesticides to stop infestations






5. Responsible for the control of pesticides - herbicides - etc






6. IPS (induced pluripotent) - ESC (embryonic stem cells - and ASC (adult stem cells






7. Protective cold- induced proteins from fish that live in very cold climates






8. Retrovirus - mediated transgenics (infect mouse embryo with retrovirus and implant) - pronuclear microinjection (introduce transgenic DNA during zygote development - inject into sperm or egg) - embryonic stem cell method (embryonic stem cells mixed w






9. Introduced as a sedative in 50's - caused birth defects - caused stricter regulations testing before drugs and pesticides were licensed






10. Pieces of DNA that can accept carry and replicate other pieces of DNA






11. US Patent and Trademark Office - only one required by the constitution - oversees patenting






12. Small group - 20-80 SAFETY - to establish dose range and side effects;






13. Injection into the organism






14. Can't be used if bacteria does not have the same post- translational modifications as eukaryotes --- no glycosylation - Can use bacteria to produce eukaryotic proteins only if the protein does not require any post- translational modifications. (Or un






15. DNA copy of an mRNA molecule expressed in a cell






16. Plasmids that are successfully engineered are white - If the gene is blue it is not effected - White colonies are inserted into the clone of DNA on Bgal site - Allows for detection of successful ligation (in vector- based gene cloning)






17. Chemically - Mechanically - New ways to take from levis






18. Composition of matter (chemical union) - Method - Article of Manufacture - Machine






19. VNTR's (variable number tandem repeats) and Subsets (STR short tandem repeats (CODIS))






20. Cells and matrixes






21. Antigens are foreign substances that stimulate an immune response






22. Must be able to define its specific utility (must know exactly what it does... specifically) - show its substantial utility (real world use) - meet the credible utility requirement (be backed by sound science) - and not be similar to something else (






23. Component of the cloning vector that allows for the selection of those bacteria colonies that actually were transformed by the vector






24. Cut small discs of leaf - culture in genetically modified agrobacter - Leaf discs are treated with hormones






25. Sequences that allows the nucleotide sequencing of cloned DNA fragments that are inserted into the plasmid






26. Laboratory technique uses a DNA polymerase from bacteria that grow in geysers - Allows for the exponential increase in the synthesis of specific segments of DNA






27. American Association for Laboratory Animal Science - oversees the treatment of laboratory animals -






28. Induced pluripotent stem cells - nuclear reprogrammed somatic cells






29. Short stretched of DNA with single base pair changes (wildtype) - Short length allows distinguishing which allele is present - Allows the probe to stay attached to the target DNA - Perfectly matched will cause a signal






30. Centers for Disease Control and Prevention - protects public health and safety - provides information about health decisions and promotes health programs - works with disease - food- borne pathogens - occupational safety.






31. 2 -00-5 -000 base pairs






32. National Institute of Health - foremost medical research center - makes important health discoveries






33. The nucleotide sequences are present in the initial mRNA transcript but are excised prior to translation






34. Advancement and regulation of agriculture - pests - plants and vet biologics - growing and farming practices






35. Fusion of two protoplast cells from different species: hybrid plant






36. The act that started the clean up of sites that had hazardous substances






37. HESC - ASCs - AFS - iPS






38. Food and Drug Administration - in charge of processed foods and medication - oversees the lab practices - phase testing of drugs - drug - food items - cosmetics






39. Genes allow for selection and identification of bacteria that has been modified (undergone recombination)






40. RFLP and PCR






41. Only 1/100th of DNA differs from person to person






42. The site for DNA replication that allows the plasmids to replicate separately from the host cell's chromosome - component of cloning vector that allows it to replicate itself inside a bacterial cell






43. To create or modulate tissue to replace or augment diseased or damaged tissue






44. Responsible for growing and farming practices






45. Stretch of DNA with recognition sequences for various types of restriction enzymes - In plasmid - Allows for range of DNA fragments to be cloned in the plasmid






46. Used in fingerprinting - Less DNA and can be slightly degraded - Extremely sensitive to contaminating DNA






47. Responsible for the standard of treatment for laboratory animals






48. Adult- dervied stem cells - Can differentiate but not as pluripotent as hESCs






49. Embryo twinning (split embyos in half) - cloned from adult DNA






50. Amniotic fluid- derived stem cells - Coaxed to become other cells