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Microbiology Board

Instructions:

Answer 50 questions in 15 minutes.
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Match each statement with the correct term.
Don't refresh. All questions and answers are randomly picked and ordered every time you load a test.

This is a study tool. The 3 wrong answers for each question are randomly chosen from answers to other questions. So, you might find at times the answers obvious, but you will see it re-enforces your understanding as you take the test each time.

1. Endotoxin
toxin from lipopolysaccharide layer of cell - heat stable - gram (-) have these
Indicate how the organisms are named - (King Paul Can Only Find Good Spices) Kingdom - Phylum - Class - Order - Family - Genus - Species
franciella tularensis - rabbit fever
1 heart disease 2 cancer 3 stroke 4 respiratory infection 5 accidents

2. Prokaryotes
1 heart disease 2 cancer 3 stroke 4 respiratory infection 5 accidents
PDD = purified protein derivative - Type IV (Delayed hypersensitivity reaction) - Ziehl Neilson staining (shows acid fast) - chest x-ray john complex
No true nucleus - no organelles (no mitochondria) ie. bacteria
Typhoid fever - salmonella enteridese -> from chicken poop

3. What is the best test for water pollution?
for serology test for all rickettsia except coxiella burnetti (Q fever) - antigen for test = proteus vulgaris
BOD Biological Oxygen Demand
dysentery
PDD = purified protein derivative - Type IV (Delayed hypersensitivity reaction) - Ziehl Neilson staining (shows acid fast) - chest x-ray john complex

4. Hypersensitivity reactions
red jelly sputum
Measles - mumps - rubella - Sabin
1 heart disease 2 cancer 3 stroke 4 respiratory infection 5 accidents
types I - IV - pneumonic = ACID (see future flash card)

5. Secondary water purification process
5. sand filtration (removes anaerobic bacteria) 6. aeration (adds oxygen) 7. chlorination (kills remaining bacteria) 8. possible flouridation
follows dermatome
dead polio vaccine - by injection - done before Sabin
C5B - C9

6. Salmonella
aka post-infectious polyradiculopathy - ascending demyelination of PNS - can be acquired after influenza vaccination or infection
T-cell growth factor - made by CD4 cells - CD4 = T-Helper cell = 'quarterback' of immune system - directs response of immune system
Typhoid fever - salmonella enteridese -> from chicken poop
161

7. Monotrichus
'old age pigment' - lipid residue - build up of lipofuscin in organs = 'brown's atrophy'
Inanimate object is a vector - hapten partial or half antigen - does not stimulate immune response - needs to bind to protein to become active
single flagella on one pole
Yellow fever

8. CDC statistics
secondary
(not research) - morbidity and epidemiology statistics
ncooked pork
bacteriophage transfers genetic material between bacteria - bacteriophage = altered virus

9. Mumps
heterophile agglutinization (Paul Bunnel) - Downey cell (don't let mono get you down) - monospot
father of pathology and germ theory - rabies vaccine - disproved spontaneous generation - pasteurization of wine - then milk
salk - rabies
Inflammation of parotids

10. What viral disease has an antigenic shift and/or drift?
Influenza
eastern equine encephalitis
causes diarrhea
dysentery

11. Commensalism
mononucleosis
2 populations living together - one benefits - the other is unaffected
Many furuncles (infected hair follicles) - 'a CAR load of furuncles' - #1 cause is staph aureus
eliminates placebo

12. What do saprophytes eat?
strawberry tongue
dead/decaying material
rubella - rabies - Hep A
Lymphocyte activating factor - made by macrophages for brain and nervous tissue

13. Primary water purification process
for gram positive - inhibits cell wall synthesis
1. filtration 2. flocculation (aluminum salts) 3. sedimentation 4. sludge digestion (add anaerobic bacteria)
psuedomonas aeruginosa - open burn infection - unsanitized hot tub - fluoresces blue under UV light from pyocyanin and fluorescein pigment
2 populations living together - both benefit

14. Lister
pre-op disinfectant
grown in candle jars culture
antisepsis - used carbolic acid to sterilize surgical instruments
fish - mouth - contaminated sushi - may cause pernicious anemia (B12) - yellow fin tuna most commonly has it

15. Leading cause of death world wide
Transmitted fecal-oral contamination - affects anterior horn (alpha motor)
1 tuberculosis 2 malaria
SERGIS: - Strep throat - Erysipelas - Rheumatic fever - Glomerulonephritis - Impetigo - Scarlet Fever
Can be in presence of absence of O2 - facultative aerobe = prefers O2 - facultative anaerobe = prefers no O2

16. Flemming
staph aureus
Affects mitral valve (aka ashkov bodies) - Can = carditis - Sammy = syndenham's chorea - Suck = subcutaneous nodule - Ethyl's = Erythema marginatum - Polyps = migratory Polyarthritis
discovered penicillin
strawberry tongue

17. Enterobacteria
Inanimate object is a vector - hapten partial or half antigen - does not stimulate immune response - needs to bind to protein to become active
Measles
causes diarrhea
influenza

18. Blue/green purulent (pus)
hepatitis associated with alcoholism
psuedomonas aeruginosa - open burn infection - unsanitized hot tub - fluoresces blue under UV light from pyocyanin and fluorescein pigment
Classified streptococcus
atypical pneumonia - mycoplasms lacks cell wall

19. Sulfa
for gram negative - competes with PABA
2 populations living together - both benefit and accomplish things that they can't do alone
discovered penicillin
Many furuncles (infected hair follicles) - 'a CAR load of furuncles' - #1 cause is staph aureus

20. Franciella
the purpose of opsonization is to enhance phagocytosis - (marks the target for strike)
STOIC: Scalded skin syndrome (exfoliative toxin) - Toxic shockOsteomyelitis - Impetigo - Carbuncles [staph aureus produces alpha toxin aka alpha hemolysin]
Catalase
franciella tularensis - rabbit fever

21. A Name for strep throa
pharyngitis
salk - rabies
Lungs - ie. tuberculosis - blastomycosis - pneumoconiosis
No true nucleus - no organelles (no mitochondria) ie. bacteria

22. Reed - Walter
Yellow fever
exposed to disease = body reacts - ie. measles - chicken pox triggers antibody production
for typhoid fever
1. find microorganism in people suffering from the disease 2. isolate and grow the microorganism in culture 3. should cause disease in a healthy individual when introduced 4. re-isolate microorganism from the inoculated people

23. Is AIDS a primary or secondary immune deficiency?
dead polio vaccine - by injection - done before Sabin
fix gross specimens
secondary
TB

24. Formaldehyde
Allergic reactions - increases in Hodgkins disease (Reed-Sternberg cells)
fix gross specimens
Measles
Came up with washing hands before delivering baby and significantly reduced puerperal fever (aka childbed fever) - this can develop into sepsis - contracted by women after childbirth

25. What are the only Gram + organisms?
161
BLCCSS (blocks) - Bacillus - Listeria monocytogenes - Clostridium - Corynebacterium diphtheriae - Staph - Strep
developed the first microscope - saw microorganisms
proved bacteria 'causes' disease - Koch's 4 postulates

26. Fomite
Inanimate object is a vector - hapten partial or half antigen - does not stimulate immune response - needs to bind to protein to become active
atypical lymphocytes
grown in candle jars culture
Steam heat under - denatures membranes - ineffective against prions

27. When examining the agar plate - describe the colony variation (morphology)
2 populations living together - both benefit and accomplish things that they can't do alone
2 populations living together - both benefit
Magic bullet - sulfa drugs - first treatment for syphilis
D= Dwarf H= Spreading M= Mucoid capsule forming - related to virulence O= compact (O vs H) R= Rough S= Smooth

28. What is the number one cause of impetigo and myelitis?
staph aureus
psuedomonas aeruginosa - open burn infection - unsanitized hot tub - fluoresces blue under UV light from pyocyanin and fluorescein pigment
serratia marcenscus
eliminates placebo

29. What will result if born without B cells at birth?

30. Flasciolopsis hepatica
In liver - contaminated aquatic plants (ie kelp) - hemoptysis
TB (inactive phase) -calcium 'jail' around mycobacterium
Controls FDA - CDC - US Public Health Services
Indicate how the organisms are named - (King Paul Can Only Find Good Spices) Kingdom - Phylum - Class - Order - Family - Genus - Species

31. Hasteurellis
Bruten's A Gamma Globulin Anemia - (primary immune deficiency)
old name
Indicate how the organisms are named - (King Paul Can Only Find Good Spices) Kingdom - Phylum - Class - Order - Family - Genus - Species
Chocolate agar aka Thayer Martin agar aka heated blood agar

32. Peritrichus

33. Diphyllobothrium latum
atypical pneumonia - mycoplasms lacks cell wall
acellular protein which denatures other proteins - cannot be autoclaved - mad cow disease
fish - mouth - contaminated sushi - may cause pernicious anemia (B12) - yellow fin tuna most commonly has it
aquatic plants - contaminated snail feces gets into plants

34. Legionella
Goodpasteur
spread by food - self limiting
river eye worm - flies - aka African Eye Worm
Legionnaire disease - water droplets - pontiac disease

35. Serratia
2 populations living together - both benefit and accomplish things that they can't do alone
red pus
Brody's abcess
BLCCSS (blocks) - Bacillus - Listeria monocytogenes - Clostridium - Corynebacterium diphtheriae - Staph - Strep

36. Councilman cells are inclusion bodies associated with...
Controls FDA - CDC - US Public Health Services
responsible for milk and milk products
Many furuncles (infected hair follicles) - 'a CAR load of furuncles' - #1 cause is staph aureus
viral hepatitis

37. Case control study
Identifies risk factors
for staph
for gram negative - inhibits protein synthesis
the purpose of opsonization is to enhance phagocytosis - (marks the target for strike)

38. Eukaryotes
In plasma cells - consists of antibodies - from chronic inflammation - and malignant disorders - associated with multiple myeloma
dysentery
for typhoid fever
True nucleus - organelles ie. human cells - protozoa like giardia and amoeba

39. Reed sternberg are inclusion bodies associated with...

40. What will you see on TB test?
PDD = purified protein derivative - Type IV (Delayed hypersensitivity reaction) - Ziehl Neilson staining (shows acid fast) - chest x-ray john complex
Regulated health products to the public
for gram negative and positive - inhibits protein synthesis - turns teeth gray or yellow brown
toxin that affects the nervous system - ie. botulism - tetanus

41. Guillene-Barre
aka post-infectious polyradiculopathy - ascending demyelination of PNS - can be acquired after influenza vaccination or infection
red pus
Lungs - ie. tuberculosis - blastomycosis - pneumoconiosis
strawberry tongue

42. Transduction
bacteriophage transfers genetic material between bacteria - bacteriophage = altered virus
whooping cough
given antibody in utero - breastfeeding - in utero = IgG - breastfeeding = IgA
dead/decaying material

43. Which vaccines are manufactured using human diploid cells?
'both' flagella are on both poles
mononucleosis
rubella - rabies - Hep A
stain for mycobacteria

44. Mutualism
Mantoux test aka tinne test aka skin prick test - PPD = purified protein derivative - delayed hypersensitivity (Type IV) - Zeihl Nielson staining (acid fast) - gohn complex in lung
Steam heat under - denatures membranes - ineffective against prions
BLCCSS (blocks) - Bacillus - Listeria monocytogenes - Clostridium - Corynebacterium diphtheriae - Staph - Strep
2 populations living together - both benefit

45. Zoonosis
small pox (variola)
Transfer of disease from animal to human - (ie anthrax gram + - sporulates)
rheumatic fever
True nucleus - organelles ie. human cells - protozoa like giardia and amoeba

46. Interleukin III
Live polio vaccine - given orally
aka post-infectious polyradiculopathy - ascending demyelination of PNS - can be acquired after influenza vaccination or infection
Made by big bone marrow - in blood and lymph system
Lungs - ie. tuberculosis - blastomycosis - pneumoconiosis

47. Type IV hypersensitivity reaction
aquatic plants - contaminated snail feces gets into plants
subunit of protein coat 'capsid' of virus particle
Many furuncles (infected hair follicles) - 'a CAR load of furuncles' - #1 cause is staph aureus
D - Delayed hypersensitivity - aka cell mediated aka cellular - T-Cell lymohocytes - this is the only one NOT antibody mediated - transplant rejection via MHC

48. Iodine
Made by big bone marrow - in blood and lymph system
No true nucleus - no organelles (no mitochondria) ie. bacteria
atypical lymphocytes
pre-op disinfectant

49. West nile virus
plasmid DNA transfer via sex pilus - bestows antimicrobial resistance
(not research) - morbidity and epidemiology statistics
eastern equine encephalitis
Tetanus by blocking inhibitory GABA and glycine - (GABA in brain - glycine in spinal cord)

50. All mycobacterium are _____
Categorizes streptococcus demonstrates the type of hemolysis (destruction of RBCs)
red pus
acid fast - M=mycobacterium - A=acid - F=fast
aka post-infectious polyradiculopathy - ascending demyelination of PNS - can be acquired after influenza vaccination or infection

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