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Molecular Biotechnology

Subject : engineering
Instructions:
  • Answer 50 questions in 15 minutes.
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  • Match each statement with the correct term.
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This is a study tool. The 3 wrong answers for each question are randomly chosen from answers to other questions. So, you might find at times the answers obvious, but you will see it re-enforces your understanding as you take the test each time.
1. LacY: Transports lactose into the cell LacZ: B- galactosidase LacA: transacetylase LacI: lacI+ cells fully inducible - lacI- were already induced and not responsive to IPTG X- gal: analog of lactose that turns blue when cleaved by lacZ product and o






2. Important to suppress mutations at 3rd position and you don't need to have a lot of stop codons; cells can be more flexible






3. Three sites recruit tRNA and forms peptidyl- tRNA bonds (E - exit; P - peptide; A - acceptor).






4. Unvarying expression of gene






5. 1. Nucleic acid hybridization: (a) bind single stranded DNA to a membrane support - (b) add single stranded labeled DNA (probe) under appropriate conditions - (c) wash the support to remove excess unbound labeled probe DNA - (d) detect the hybrid seq






6. In E. coli - DNAP III can unwind DNA (helicase) and replicate both strands of DNA. It also has proofreading activity and corrects mistakes 3' to 5' exonuclease






7. Functions: enzymes - regulation - structural - cellular functions Polymers of amino acids and connected by peptide bonds. Can fold into complex structures.






8. Genes for products that are required at all times.






9. Operator site; araC bound at this site can simultaneously bind to the araI site to repress transcription from Pbad promoter






10. Reverse Transcriptase






11. The ribosome translating the leader peptide arrives at the two tryptophan codons and has to wait for tryptophan. During this time - RNAP continues to transcribe. Stem loop between 2 and 3.






12. Inducer site; araC bound at this site can simultaneously bind to the araO2 site to repress transcription from the Pbad promoter. In the presence of arabinose - araC bound at this site helps to activate expression of Pbad promoter.






13. Search for site to start transcription - unwind DNA; -35 region and pribnow region (-10 region).






14. TrpE through trpA are five enzymes that catalyze the synthesis of the amino acid tryptophan from chorismic acid. If the cell has enough tryptophan - then it doesn't need to waste energy transcribing this mRNA. In the presence of tryptophan - the oper






15. Comprised of >50 proteins associated with rRNA units. Site of protein synthesis and binds mRNA and finds protein synthesis initiation sites. It also binds aa- tRNA and catalyzes peptide bond formation.






16. 1. mRNA - template for protein synthesis 2. tRNA - carrier of amino acid (the adaptor)3. aminoacyl- tRNA synthetase - pairs tRNA with the cognate amino acid - needs ATP 4. ribosome - site of protein synthesis - read in three frames - start codon is A






17. In the presence of glucose and lactose - bacteria grows first on glucose - then growth levels off - and starts growing on lactose. You have diauxie growth because (1) CAP helps recruit RNAP. in the presence of glucose - CAMP is low so it can't bind t






18. When half DNA strand has been denatured. Determined by GC content (triple bond)






19. 1. Initiation: unwind DNA at the origin of replication (ori) - bidirectional replications; regulated as required for cell division 2. Elongation: requires RNA primer to replicate 3. Termination: signaled by Ter sequence






20. 4. Cells + organelle 3. Supermolecular complexes 2. Macromolecules 1. Monomeric units






21. The process of increasing the expression of inducible genes






22. Replication > DNA > Transcription > RNA > Translation > Protein






23. Multiple effects from a single gene






24. The process of decreasing the expression of inducible genes






25. Binds to CAP binding site. In conjunction with araC bound with arabinose - it assists RNAP in binding to the Pbad promoter






26. C - N - O - H make up 99% cell weight - 70% is water






27. When arabinose is absent - there is no need to express the structural genes. AraC does this by binding simultaneously to araI and araO2 - making a looped DNA. This blocks access to Pbad promoter. AraC is an autoregulator of its own expression and the






28. In prokaryotes - related genes often arrayed in tandem. A unit of bacterial gene expression and regulation - recognized by a regulator gene product






29. Ribosome doesn't stop at trp codons and stem loop forms between 3 and 4. RNAP stops prematurely (attenuated)






30. The process in which an exact copy of the double strand DNA is made. It is a templated process and occurs from 5' to 3' end. DNAP - RNA primer; semiconservative (each strand is a template for the replication of the complementary strand)






31. Gene products decrease in concentration under particular molecular circumstances






32. 1. mRNA: encodes genetic information 2. tRNA: transfer RNA - involved in protein synthesis (DNA to amino acids) 3. rRNA: ribosome RNA - involved in protein synthesis (polypeptide formation) 4. Ribozymes and RNAi - Can store genetic information and ca






33. A cell that contains a nucleus and membrane bound organelles






34. The small ribosomal subunit binds to 5'-G cap on processed mRNA (no RBS) - uses met instead of fmet for initiation; monocistronic translation






35. Eukaryotic. mRNA that codes for one protein






36. A reading frame without termination codon among 50 or more codons. Usually correspond to genes that encode proteins






37. A small catabolite molecule. Its level is determined by the level of glucose in the cell where glucose controls the rate of cAMP formation with ATP. When there is high glucose - there is low levels of cAMP. cAMP activator protein (CAP) has to bind cA






38. A haploid organism that is diploid for a small region of the chromosome (partial diploid)






39. The first two bases of the codon always form strong Watson -Crick base- pairing. The first base in the anticodon determines the number of codons a tRNA can recognize. The first position in anticodon is often modified to inosine to facilitate wobble b






40. 1. LacI- makes an internal inducer -- NO. Found that lacI- doesn't dominate over lacI+ and is not always constitutive. 2. LacI- is a repressor protein -- YES. LacI+ dominates over lacI- because when both are together - lac operon is inducible. LacI m






41. Codes for three enzymes needed to catalyze the metabolism of arabinose. The operon is regulated by araC gene product.






42. A segment of DNA molecule contains the information required for synthesis of a functional biological product






43. Select correct ribonucleotides; loss of sigma factor - transcription bubble - no need for primers






44. Gene products increase in concentration under particular molecular circumstances






45. Release DNA - rewind DNA - release RNA; stop signals or rho mediated termination (hairpin is a palindromic GC- rich region followed by an AT- rich region; Rho is a termination factor that binds to nascent RNA) RNAP has sigma factor that recognizes pr






46. Need to remove introns before changing into mRNA - then take mRNA out of the nucleus. Has 3 RNAP (RNAP I synthesizes rRNA - II synthesizes mRNA - III synthesizes tRNA and small rRNA). Transcription factors are similar to sigma factors.






47. Polymerase binds to lac promoter weakly by itself and results in low levels of transcription even in the absence of lacI. The activator recruits the polymerase to the promoter region and increases its affinity for the promoter






48. A templated process just like in DNA replication and there is no processing steps.






49. Determines amino acid selection. A noncognate amino acid charge incorrectly to the tRNA will be inserted into the protein. Introduce new amino acid by using tRNA for UAG.






50. 1. Synthesis of commercial products by recombinant organisms 2. Biopolymers 3. Bioremediation 4. Biomass utilization